Table 3—

Housekeeping gene(HKG) expression stability# as determined by three different Visual Basic for Applications applets

geNormBestKeeperNormFinder
GeneMean MGener+GeneStability value
PPIA/RPLP00.416PPIA0.988PPIA0.111
PGK10.984PGK10.129
GNB2L10.449B2M0.956RPLP00.169
PGK10.488GUSB0.953GNB2L10.209
GUSB0.528GNB2L10.953GUSB0.246
B2M0.564RPLP00.952B2M0.258
RNR10.615RNR10.925RNR10.323
GAPDH0.684ACTB0.915ACTB0.396
ACTB0.716GAPDH0.913GAPDH0.405
TFRC0.903TFRC0.847TFRC0.594
  • M: gene expression stability. PPIA: cyclophilin A (or peptidylprolyl isomerase A) gene; RPLP0: acidic ribosomal protein gene; GNB2L1: guanine nucleotide-binding protein, β-peptide 2-like 1, gene; PGK1: phosphoglycerokinase gene; GUSB: β-glucuronidase gene; B2M: β2-microglobulin gene; RNR1: 18S ribosomal RNA gene; GAPDH: glyceraldehyde-3-phosphate dehydrogenase gene; ACTB: β-actin gene; TFRC: transferrin receptor gene. #: genes listed from most to least stable for all three methods; : stability value; +: all 10 candidate HKGs were highly correlated and were combined to calculate the BestKeeper index, which was then used to determine the correlation between each HKG and the index.