Effect of transfection on interleukin(IL)-4-receptor (R)α and IL-2Rγ expression at the cell surface in BEAS-2B cells
| Transfection | Mean fluorescence intensity | |
| IL-4Rα | IL-2Rγ | |
| None | 3.21±0.20# | 8.01±0.19# |
| None | 4.79±0.20¶ | 14.80±0.11+ |
| Effectene reagent | 6.34±0.11§ | 14.47±0.09 |
| Poly(I) 100 ng·well−1 | 6.35±0.24§ | 14.56±0.22 |
| Poly(dIdC) 100 ng·well−1 | 6.21±0.64§ | 13.89±0.46 |
| Poly(IC) 1 ng·well−1 | 7.03±0.19§,ƒ | 17.45±0.15§,ƒ |
| Poly(IC) 10 ng·well−1 | 9.42±0.15§,ƒ | 19.88±0.14§,ƒ |
| Poly(IC) 100 ng·well−1 | 11.20±0.23§,ƒ | 24.47±0.48§,ƒ |
Data are presented as mean±sd from three sets of data. Poly(I): poly inosinic-acid; Poly(dIdC): poly deoxyinosinic-deoxycytidyric acid; Poly(IC): poly inosinic-cytidyric acid. #: control immunoglobulin (Ig)G; ¶: anti-IL-4Rα; +: anti-IL-2Rγ; §: p<0.01 compares the value in nontransfected cells labelled with anti-IL-4Rα or anti-IL-2Rγ; ƒ: p<0.01 compares the value in poly(I) transfected cells.