Primers and TaqMan probes
| Endothelin-1 |
| Forward 5′- CTCCTGCTCTTCCCTGATGG -3′ |
| Reverse 5′- TGGCACACTGGCATCTATCC -3′ |
| TaqMan probe 5′(FAM)- TTCTGCCACCTGGACATCATTTGGG -(TAMRA)3′ |
| Interleukin-1β |
| Forward 5′ GGTTTCTGAAGCAGCCATGG -3′ |
| Reverse 5′ GATTTGCAGCTGGATGCTCC -3′ |
| TaqMan probe 5′ (FAM)- AAAGAGATGAAGTGCTGCACCCAAAACCTG -(TAMRA)3′ |
| Hypoxanthine-guanine-phosphoribosyl-transferase |
| Forward 5′- CGGCTCCGTTATGGCG -3′ |
| Reverse 5′- GGTCATAACCTGGTTCGTCATCA -3′ |
| TaqMan probe 5′(FAM)- CGCAGCCCCAGCGTCGTGATTA -(TAMRA)3′ |