Analysis of silver-stained proteins separated by high-resolution two-dimensional polyacrylamid gel electrophoresis (2D-PAGE)
| Control | CF | p-value | |
| Subjects n | 8 | 17 | |
| Total number of proteins in 80 μg* | 190±63 | 189±50 | 0.977 |
| Number of proteins with MW ≤20 kD of total protein %* | 14.5±3.9 | 40.3±7.8 | <0.0001 |
| Vol% of proteins with MW ≤20 kD of total protein* | 7.7±1.1 | 49.0±2.6 | <0.0001 |
| Vol% of α1-antitrypsin of total protein+ | 0.5±0.2 | 9.3±1.9 | 0.0051 |
| Vol% of albumin of total protein+ | 26.3±3.0 | 0.3±0.3 | <0.0001 |
Results are expressed as the mean±sem for n independent determinations. Comparisons were made for continuous variables by an unpaired two-tailed t-test. *: these data result from the analysis of silver-stained 2D PAGE gels by Melanie 2.1 software. Vol% of a given protein spot is a function of its area and its intensity referred to the volume of all protein spots on the gel. +: proteins were identified by Western blot analysis of bronchoalveolar lavage proteins after separation by two dimensional electrophoresis under reducing conditions. Corresponding protein spots were localized on silver-stained gels run in parallel. Analysis was performed as described above from the silver-stained gels.