Anaplastic lymphoma kinase (ALK) immunohistochemistry (IHC), fluorescence in situ hybridisation (FISH) and quantitative reverse transcriptase PCR (qRT-PCR) data in ALK FISH-positive and -negative tumours
| ALK FISH-positive tumours | ALK FISH-negative tumours | p-value | |
| Tumours | 140 | 400 | |
| IHC clone 5A4 | |||
| Samples | 120 | 375 | |
| Mean positive cells % | 87 | 12 | <0.0001 |
| Mean staining intensity | 2.3 | 1.7 | <0.0001 |
| Mean classic score | 213 | 81 | <0.0001 |
| Mean H-score | 211 | 81 | <0.0001 |
| IHC clone D5F3 | |||
| Samples | 75 | 256 | |
| Mean positive cells % | 92 | 25 | <0.0001 |
| Mean staining intensity | 2.6 | 1.2 | <0.0001 |
| Mean classic score | 255 | 77 | <0.0001 |
| Mean H-score | 256 | 81 | <0.0001 |
| FISH | |||
| Rearranged cells % | 63 (21–100) | 2 (0–9) | <0.0001 |
| Mean nuclei analysed | 90 | 86 | |
| qRT-PCR | |||
| Mean RNA extracted ng·μL−1 | 139 | 174 | 0.06 |
| ALK variant Ct | 35 (26–44) | 30 (26.6–35) | 0.05 |
| Reference gene Ct | 26 (21–37) | 26 (21–35) | ns |
| No ALK variant detected | 50 (36) | 378 (95) | <0.0001 |
| Variant 1 | 33 (24)# | 1 | <0.0001 |
| Variant 2 | 9 (6) | 0 | <0.0001 |
| Variant 3a/b | 29 (21) | 0 | <0.0001 |
| Variant 5 | 3 (2)¶ | 0 | 0.03 |
| Variant 7 | 1 (1) | 0 | ns |
| Not available | 14 (10) | 9 (2) | 0.0004 |
| Not interpretable | 5 (4) | 10 (2) | ns |
Data are presented as n, mean (range) or n (%), unless otherwise stated. Ct: cycle threshold; ns: nonsignificant. #: one with 2 and 3a; ¶: one with 3a and one with 7J.