TABLE 2 Primer and probe sequences (5′→3′)
TargetSequence[Ref.]
18S rRNA[24]
 Forward primerCGCCGCTAGAGGTGAAATTCT
 Reverse primerCATTCTTGGCAAATGCTTTCG
 ProbeFAM-ACCGGCGCAAGACGGACCAGA-TAMRA
RV[24]
 Forward primerGTGAAGAGCCSCRTGTGCT
 Reverse primerGCTSCAGGGTTAAGGTTAGCC
 ProbeFAM-TGAGTCCTCCGGCCCCTGAATG-TAMRA
IFN-λ2/3[25]
 Forward primerCTGCCACATAGCCCAGTTCA
 Reverse primerCTGCCACATAGCCCAGTTCA
 ProbeFAM-TCTCCACAGGAGCTGCAGGCCTTTA-TAMRA
IFN-β[24]
 Forward primerCACGGATACAGAACCTATGG
 Reverse primerACGAACAGTGTCGCCTACTA
 ProbeFAM-TCAGACAAGATTCATCTAGCACTGGCTGGA-TAMRA
LDLR[26]
 Forward primerTGACAATGTCTCACCAAGCTCTG
 Reverse primerCTCACGCTACTGGGCTTCTTCT
 ProbeFAM-CTGCCAGCAACGTCG-TAMRA
TLR3[24]
 Forward primerAAATTAAAGAGTTTTCTCCAGGGTGTT
 Reverse primerATTCCGAATGCTTGTGTTTGC
 ProbeFAM-TTTGGCCTCTTTCTGAACAATGTCCAGC-TAMRA
TLR2[27]
 Forward primerTTTCACTGCTTTCAACTGGTA
 Reverse primerTGGAGAGGCTGATGATGAC
 ProbeFAM-CAAGACCCACACCATCCACAA-TAMRA
RIG-I[24]
 Forward primerCCAAGCCAAAGCAGTTTTCAA
 Reverse primerCACATGGATTCCCCAGTCATG
 ProbeFAM-TTGAAAAAAGAGCAAAGATATTCTGTGCCCGAC-TAMRA
MDA5[24]
 Forward primerGATTCAGGCACCATGGGAAGT
 Reverse primerAGGCCTGAGCTGGAGTTCTG
 ProbeFAM-GGGATGCTCTTGCTGCCACATTCTCTT-TAMRA
Viperin[12]
 Forward primerCACAAAGAAGTGTCCTGCTTGGT
 Reverse primerAAGCGCATATATTTCATCCAGAATAAG
 ProbeFAM-CCTGAATCTAACCAGAAGATGAAAGACTCC-TAMRA
OAS[12]
 Forward primerCTGACGCTGACCTGGTTGTCT
 Reverse primerCCCCGGCGATTTAACTGAT
 ProbeFAM-CCTCAGTCCTCTCACCACTTTTCA-TAMRA
MxA[12]
 Forward primerCAGCACCTGATGGCCTATCAC
 Reverse primerCATGAACTGGATGATCAAAGG
 ProbeFAM-AGGCCAGCAAGCGCATCTCCAG-TAMRA
IL-8[24]
 Forward primerCTGGCCGTGGCTCTCTTG
 Reverse primerCCTTGGCAAAACTGCACCTT
 ProbeFAM-CAGCCTTCCTGATTTCTGCAGCTCTGTGT-TAMRA
IL-6[12]
 Forward primerCCAGGAGCCCAGCTATGAAC
 Reverse primerCCCAGGGAGAAGGCAACTG
 ProbeFAM-CCTTCTCCACAAGCGCCTTCGGT-TAMRA
IP-10[24]
 Forward primerCCATTCTGATTTGCTGCCTTATC
 Reverse primerGCAGGTACAGCGTACAGTTCT
 ProbeFAM-CTGACTCTAAGTGGCATTCAAGGAGTACCTCTCTC-TAMRA

  • rRNA: ribosomal RNA; RV: rhinovirus; IFN: interferon; LDLR: low-density lipoprotein receptor; TLR: Toll-like receptor; RIG: retinoic acid-inducible gene; MDA: melanoma differentiation-associated protein; viperin: virus inhibitory protein, endoplasmic reticulum-associated, IFN inducible; OAS: 2′,5′-oligoadenylate synthase; IL: interleukin; IP-10: 10-kDa IFN-γ-induced protein; FAM: 6-carboxyfluorescein; TAMRA: tetramethylrhodamine.