Protocol | Mean PCR cycle |
Direct testing of CSF | |
0.5 mL | 1.9 |
1.0 mL | 1.4 |
1.5 mL | Referent |
2.0 mL | 1.5 |
Sample reagent:sample ratio | |
1:1 | 1.8 |
2:1 | 0.1 |
3:1 | 0.2 |
CSF centrifuged | |
4 mL | 0.6 |
8 mL | 0.2 |
Replicates of referent sample# | 1.7 |
Data are presented as the threshold difference. #: direct testing of 1.5 mL CSF.