RT Journal Article
SR Electronic
T1 Age-dependent influence of ADRB2 gene polymorphisms on asthma severity in Serbian population
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P3834
VO 44
IS Suppl 58
A1 Natasa Petrovic-Stanojevic
A1 Aleksandra Topic
A1 Aleksandra Nikolic
A1 Marija Stankovic
A1 Vesna Dopudja-Pantic
A1 Branislava Milenkovic
A1 Dragica Radojkovic
YR 2014
UL http://erj.ersjournals.com/content/44/Suppl_58/P3834.abstract
AB Background: Beta 2-adrenergic receptor is expressed in airway smooth muscle and induces bronchial relaxation. It is assumed that two common investigated polymorphisms of the beta2-adrenergic receptor gene (ADRB2), Arg16Gly and Gln27Glu are responsible for the susceptibility to asthma. Aims: We investigated whether ADRB2 polymorphisms affect severity of asthma in population of Serbian asthmatics in relation of age. Asthma severity were evaluated based on Asthma Control Test (ACT) and results of spirometry testing (FEV1 percentage). Methods.The study included a group of 162 patients with asthma, divided in two age groups, (<50 year old, n=105 and >50 year old, n=57). Genotyping of Arg16Gly and Gln27Glu polymorphisms was performed by direct sequencing of PCR products. Results. Younger 16ArgGly heterozygotes showed significant higher values for FEV1 and ACT than both 16GlyGly (p=0.05 and p=0.045, respectively) and 16ArgArg (p=0.042 and p=0.024) homozygotes. In addition, younger asthmatic with 27GluGlu genotype showed tendency to have higher FEV1 value than 27GlnGln, but probability was near to significant (p=0.068). Younger carriers with combined genotypes 16GlyGly/27GlnGln showed significant lower FEV1 and ACT than 16Gly-Gly/27Glu-Glu (p=0.044) and 16ArgGly/27GlnGln (p=0.007). Neither the Arg16Gly nor Gln27Glu polymorphisms were not associated with asthma severity in older asthmatics. Conclusion. This is the first study which suggests that Serbian asthmatics younger than 50 years with combination of genotypes 16GlyGly/27GlnGln showed worse lung function and ACT. This result may be of clinical interest in prevention of disease severity.