PT  - JOURNAL ARTICLE
AU  - Tatiana Surovenko
AU  - Ludmila Zgeleznova
TI  - House dust mites (HDM) natural exposure and α1 –acid glycoprotein (α1-AGP) level in expired air condensates (EAC) and nasal lavage fluid (NLF) of children with bronchial asthma (BA) and allergic rhinitis (AR)
DP  - 2014 Sep 01
TA  - European Respiratory Journal
PG  - P1095
VI  - 44
IP  - Suppl 58
4099  - http://erj.ersjournals.com/content/44/Suppl_58/P1095.short
4100  - http://erj.ersjournals.com/content/44/Suppl_58/P1095.full
SO  - Eur Respir J2014 Sep 01; 44
AB  - α1-AGP is acute phase inflammation protein. We assume it plays important role in allergic inflammation of airways. The environmental factors promoting its secretion in children are not well defined. The aim of work was to find out the relationship between the levels of HDM in the children beds and α1-AGP level in EAC and NLF of children with BA and AR. After a cross-sectional study of preschool and school children which included ISAAC questionnaire and skin testing for dust mite sensitization we made repeated measurements of HDM in mattresses and α1-AGP in EAC and NLF by immunoassay. Dust samples from mattresses of patients homes (54-BA, 48- AR) were collected. The average number of HDM were 202,6+87 per gr . 86,4+ 5,33 % of beds had HDM level from 100 to 500, 3,2+2,4% –less 100, and 10,4+2,9% from 500 to 1000 per gr. α1-AGP was detected in all EAC-and NLF-samples. Significant differences were observed in α1-AGP level in BA and AR patients and in the controls. More higher level of a1-AGP in the EAC of BA patients was observed (21,4±0,36ng/ml). Patients with AR had high level of this protein in NLF(196,4±26,3 ng/ml). Control results were in EAC-4,4±0,6; in NLF-88,7±7,6ng/ml. Relationship between α1-AGP and HDM level was found. High indices were revealed in NLF of patients with number of HDM in mattresses more 500/gr. Conclusions: It confirms HDM natural exposure increases secretion of α1-AGP in airways of HDM sensitized children with AR and BA and α1-AGP could be used as biomarker of allergic inflamation of airways.