RT Journal Article
SR Electronic
T1 The role of cyclooxygenas-2 in the protection against apoptosis in vascular endothelial cells induced by cigarette smoking
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P3830
VO 44
IS Suppl 58
A1 Huihui Zeng
A1 Yan Chen
A1 Yanfang Pei
YR 2014
UL http://erj.ersjournals.com/content/44/Suppl_58/P3830.abstract
AB Background: Apoptosis has been demonstrated to be an important upstream event in the pathogenesis of chronic obstructive pulmonary disease (COPD). Cyclooxygenase-2 (COX-2) seems to be biologically relevant in COPD. However, the role of COX-2 in the endothelial apoptosis induced by CSE remains to be elucidated.Methods:Surgical specimens were obtained from 20 patients with COPD, smoking controls and nonsmoking controls. The apoptotic index and COX-2 protein expression were detected in the lung tissues and HUVECs ( human vascular endothelial cell line) under exposure to varied concentrations of CSE as well as 5.0% CSE for varied durations. Repeatedly, the apoptosis rate and COX-2 expression in HUVECs under 5.0% CSE were examined after exposing to varied concentrations of celecoxibResults:Significantly increased apoptotic index and expression of COX-2, were found both in the patients with COPD and smoking controls compared with nonsmoking controls. The CSE induced apoptosis in HUVECs are dose-dependent and time-dependent manners. The COX-2 was slightly expressed in the cells after exposing to 5% CSE for 3 and 6 hours, and markedly expressed after the exposure for 9 and 12 hours, but vanished after 24 hours of the exposure. Of interest, with the completely block of the COX-2 expression by celecoxib at 50.0μmol/L, the apoptosis rate was markedly increased again in HUVECsunder exposure to 5.0% CSE.Conclusion:Endothelialapoptosis and the expression of COX-2 protein were increased in both COPD patients and CSE-induced endothelial cells. Of interest, it seems that the COX-2 probably had a protective role against the endothelial apoptosis induced by cigarette smoking.