PT  - JOURNAL ARTICLE
AU  - Mustafa Calik
AU  - Guler Yavas
AU  - Saniye Goknil Calik
AU  - Cagdas Yavas
AU  - Esin Celik
AU  - Özlem Ata
AU  - Hidir Esme
TI  - An electron microscopic study of amelioration of radiation-induced lung injury by halofuginone
DP  - 2014 Sep 01
TA  - European Respiratory Journal
PG  - P3916
VI  - 44
IP  - Suppl 58
4099  - http://erj.ersjournals.com/content/44/Suppl_58/P3916.short
4100  - http://erj.ersjournals.com/content/44/Suppl_58/P3916.full
SO  - Eur Respir J2014 Sep 01; 44
AB  - Purpose: To evaluate the effects of Halofuginone (H) on radiation-induced lung injury (RILI).Methods: Wistar-Albino rats were treated with 12 Gy of thoracic irradiation. H was applied intraperitoneally with daily doses of either 2.5 or 5 μg. At 6th and 16th weeks of radiotherapy (RT), 5 rats from each group were sacrificed. The lung tissues were dissected for both light and electron microscopy.Results: The inflammation, fibrosis and transforming growth factor (TGF)-β scores of all study groups were significantly different at 6th and 16th week of RT (p< 0.001 for all). H, either used with lower or higher doses ameliorated the radiation-induced pulmonary fibrosis at 6th and 16th week of RT (p< 0.001 for all). In parallel to this finding, the differences between the TGF- β scores of RT only and RT+H groups' 6th and 16th week of RT were significantly different (p< 0.001 for all). The inflammation, fibrosis and TGF- β scores of RT only group were deteriorated at 16th week of RT when compared to 6th week findings (p< 0.001 for all). On the other hand only the TFG- β scores of RT+2.5 μg H group (p<0.001), and the fibrosis scores of RT+5 μg H group were significantly different at 6th and 16th weeks of RT. Electron microscopy revealed that at 6th week of RT, all the RT groups independent from H application had similar findings. The ultrastructural finding of RT+ 2.5 μg H group was same with RT only group; however the rats from 5 μg H+RT group had similar findings with 6th 5 μg H+RT group at 16th week of RT.Conclusion: H either used in lower or higher doses may ameliorate the RILI. The most prominent improvement was observed in higher group after long term follow-up.