TY - JOUR T1 - Inducible nitric oxide synthase polymorphism in asthmatic patients JF - European Respiratory Journal JO - Eur Respir J VL - 44 IS - Suppl 58 SP - P3837 AU - Margarida Cortez e Castro AU - Andreia Matos AU - Joana Ferreira AU - Ridhi Prabhudas AU - Manuel Bicho Y1 - 2014/09/01 UR - http://erj.ersjournals.com/content/44/Suppl_58/P3837.abstract N2 - Aim of the study: To analyze the association between inducible nitric oxide synthase (iNOS) gene polymorphism iNOS: + 14C T (Ex16 + 14C T) rs 2297518; with asthma severity compared with a control group of healthy blood donors.Material and Methods:Asthmatic patients (n= 100) compared with a control group (n=72); polymorphisms analyzed by PCR-RFLP. Control of asthma assessed by (ACQ7 and PAQLQ). Statistical analysis with PASW version 18 establishing a significance level of p< 0.05.Results: Mean age of 100 asthmatics: 38.1±18.6 years(7 - 86 years); 65 females and 35 males .Mean age of control-group (n=72) was 41.68±11.71 years (19 - 69 years); 25 females and 47 males. In asthmatics the frequencies of Allele C 0.81 and of Allele T 0.19; in controls: 0.92 and 0.08 respectively. There is statistical difference between these groups (p=0.003).Being the allele T more frequent in asthmatics. Genotypes in the asthmatics- CC: 66%; CT: 30%; TT: 4%; in control group- CC: 86.1%; CT:12.5%; TT: 1.4%. There is statistical difference between these groups (p=0.010). When we associate genotypes (CC vs CT+TT; p = 0.003), the genotypes who express allele T were more frequent in the asthma-group.The risk (OR) associated is 3.338 (CI 95% [1.524 - 7.313], (p =0.003).In asthmatics, there is no statistical differences in genotype frequencies (p>0.05) between: atopics and non atopics; controlled and uncontrolled asthma; males and females; and in the different age-groups.Conclusion: With this results we might conclude that Inducible nitric oxide synthase (iNOS ) polymorphism(Ex16 + 14C T), could be a risk factor for asthma, we also concluded that the genotypes who express the allele T are more prevalent in asthmatics. ER -