RT Journal Article
SR Electronic
T1 Both intranasal and intraperitoneal administration of human tubal mesenchymal stem cells (htMSC) ameliorates experimental COPD model
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P1792
VO 44
IS Suppl 58
A1 Jean Pierre Peron
A1 Gabriel Moraes
A1 Auriléia Brito
A1 Elaine Silveira
A1 Benoni Rodrigues, Jr
A1 Rodolfo Avelino
A1 Bruno Franco
A1 Giulia Rossi
A1 Mayra Pellaty
A1 Mayana Zats
A1 Lucila Evangelista
A1 Silvio Halpern
A1 Rodolfo Vieira
A1 Ana Paula Oliveira
YR 2014
UL http://erj.ersjournals.com/content/44/Suppl_58/P1792.abstract
AB Chronic Obstructive Pulmonary Disease (COPD) is poorly managed with currently available therapies. Accordingly, the use of mesenchymal stem cells (MSCs) is an innovative and accessible treatment of pulmonary chronic diseases. These cells have an important therapeutic potential, being immune suppressive, anti-fibrogenic, promoters of tissue progenitor cells, anti-apoptotic. Thus, we investigated the effect of human tubal-derived MSCs (htMSCs) in the treatment of cigarette smoke-induced pulmonary inflammation in mice. To induce COPD C57Bl/6 mice were submitted to cigarette smoke for 75 days (2 times/day). Next, animals were treated with htMSCs by intraperitoneal (COPD+htMSC(ip) group) or intranasal route (COPD+htMSC(in) group) 7 and 15 days before the experiment on day 76th, when mice were euthanized for morphologic and functional analysis of the lungs. Bronchoalveolar lavage (BAL) analysis showed that COPD+htMSC(ip) and COPD+LLL+htMSC(in) groups had a significant decrease in total cells, neutrophils and macrophages when compared to COPD group. The number of lymphocytes was more reduced in COPD+htMSC(ip) than COPD+htMSC(in) group. Both routes reduce mucous secretion, collagen deposition and alveolar enlargement, associated with reduced IL-1beta, IL-6, KC and TNF-a in BAL. More interestingly, lung expression of NF-KB and NFAT were both reduced in the experimental groups. However, the lung expression of IL-10 is increased only in COPD+htMSC(in) group. These results demonstrate that both htSMSC routes have anti-inflammatory effect on COPD, and thus may be considered as possible therapeutic approaches. Financial Support: UNINOVE, FAPESP, CNPq.