RT Journal Article
SR Electronic
T1 Effect from conditioned medium of mesenchymal stem cells and antioxidant enzymes on the processes of regeneration in the trachea
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P885
VO 44
IS Suppl 58
A1 A. Temnov
A1 V. Novoselov
A1 A. Volkova
A1 E. Karaduleva
A1 A. Skifas
A1 S. Soodaeva
A1 I. Klimanov
A1 A. Chucalin
YR 2014
UL http://erj.ersjournals.com/content/44/Suppl_58/P885.abstract
AB Lately a number of studies have testified the efficiency of mesenchymal stem cells (MSC) in the treatment of patients having the symptoms of systemic inflammation and acute organ impairment. In present work we studied the effect from the application of conditioned medium of mesenchymal stem cells on regeneration processes in the tracheal epithelium following thermal inhalation injury of the upper respiratory tract of rat, caused by hot vapor (70 oC) inhalation. In present model massive death of all types of cells of the tracheal epithelium is observed (30-100 % of the epithelium surface), with a maximum in cell death on day 3 after the inhalation injury, and a partial recovery of the epithelium in 7 days after the injury. The application of MSC medium in the burnt trachea resulted in a decreased number of dead cells by 10-20 % in the first day after the burn as compared to the control. An increased rate of epithelium recovery was observed in the next days, and 60-70 % of the epithelium surface was restored by the seventh day. In case of simultaneous application of MSC medium and Prx6 into the trachea in the first day after the burn, the amount of dead cells reduced by 50-60 %, and almost intact epithelium was observed by the third day after the burn. Thus, for the neutralization of oxidative stress in this model we have demonstrated the synergism of effects from the paracrine factors present in the conditioned medium of mesenchymal stem cells, which are represented by different types of cytokines with expressed antiapoptotic and progenerative properties.