RT Journal Article SR Electronic T1 PCR identification of HPV from recurrent respiratory papillomatosis JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP P589 VO 44 IS Suppl 58 A1 Metodi Kunchev A1 Toma Avramov A1 Ilya Tsekov A1 Antoaneta Manolova YR 2014 UL http://erj.ersjournals.com/content/44/Suppl_58/P589.abstract AB INTRODUCTION: Recurrent respiratory papillomatosis (RRP) is the most common benign neoplasm affecting the larynx and upper respiratory tract. The RRP has been associated with an infection caused by human papillomavirus (HPV), mainly types 6 and 11, the latter being associated with more severe RRP.MATERIAL AND METHOD: The present study evaluates the occurrence of HPV types in a group of 30 Bulgarian patients with RRP. The clinical records of the patients were reviewed, and RRP was classified as non-aggressive or aggressive. Laryngeal biopsies were taken and investigated for presence of HPV DNA using polymerase chain reaction (PCR) with a set of type-specific primers for HPV- 6, 11, 16, 18, 31, 33, 35, 39, 45, 52, 56, 58, 59 and 66. RESULTS: HPV type 6 and 11 was confirmed in all of the cases, where 19 patients had HPV-11, 7 patients - HPV-6 and double positivity for HPV types 6 and 11 was documented in 4 cases.CONCLUSION: The findings suggest that a more aggressive clinical course of RRP develops when HPV-11 infection is present. We believe that the routine application of molecular techniques such as PCR for detection and analysis of HPVs in patients with RRP has diagnostic and prognostic significance.