TY - JOUR T1 - Abnormal vitamin D status negatively affects lung function and inflammation in smoking mice JF - European Respiratory Journal JO - Eur Respir J VL - 44 IS - Suppl 58 SP - P868 AU - Nele Heulens AU - Nele Cielen AU - Elien De Smidt AU - Karen Maes AU - Chantal Mathieu AU - Ghislaine Gayan-Ramirez AU - Wim Janssens AU - Hannelie Korf Y1 - 2014/09/01 UR - http://erj.ersjournals.com/content/44/Suppl_58/P868.abstract N2 - Background: Increasing evidence suggests a role for vitamin D (vitD) in chronic obstructive pulmonary disease (COPD). Our aim was to investigate the effect of vitD status on disease progression, airway inflammation and alveolar macrophage (AM) antimicrobial function in a mouse model of smoke-induced COPD.Methods: Mice that received a diet containing either low-, normal- or high vitD were daily exposed to cigarette smoke or ambient air. After 6 and 12 weeks of smoking, lung function and inflammatory cells in the bronchoalveolar lavage fluid were determined. The ex vivo phagocytic- and oxidative burst capacity of AM following interaction with E. coli was assessed.Results: Mice with low- or high vitD levels showed an earlier increase in total lung capacity and compliance with smoking compared to mice with normal vitD levels. Similarly, mice with an abnormal vitD status featured an exacerbated neutrophilic inflammation after 6 and 12 weeks of smoke exposure. Smoking characteristically resulted in a significant decrease of AM phagocytic- and oxidative burst function. AM of mice with abnormal vitD levels showed a further decrease in oxidative burst capacity, but bacterial engulfment was not further affected.Conclusion: Insufficient- or overabundant vitD levels lead to an early onset of smoke-induced emphysema, elevated neutrophilic inflammation and a more pronounced defective oxidative burst capacity of AM. ER -