TY - JOUR T1 - Effect of chrysin on airway inflammation induced by cigarette smoke in mice JF - European Respiratory Journal JO - Eur Respir J VL - 44 IS - Suppl 58 SP - P3840 AU - Hao Wang AU - Diandian Li AU - Liuqun Jia AU - Lian Liu AU - Yanqiu Wu AU - Caishuang Pang AU - Yongchun Shen AU - Fuqiang Wen Y1 - 2014/09/01 UR - http://erj.ersjournals.com/content/44/Suppl_58/P3840.abstract N2 - Objective: Cigarette smoke(CS) is known to cause airway inflammation. The present study aimed to investigate the effects of chrysin on airway inflammation induced by CS in mice, and explore the possible mechanism.Methods: Mice were exposed to CS for 6 days per week for 4 weeks, low-dose(5 mg/kg·d) or high-dose(10 mg/kg·d) chrysin was intraperitoneally injected prior to CS exposure. TNF-α, IL-1β and IL-8 levels in bronchoalveolar lavage fluid(BALF) were detected by ELISA. Myeloperoxidase(MPO) levels in lung homogenate were tested by chemical chromatometry. The expressions of phospho-Extracellular signal-Regulated Kinase(p-ERK) and phospho-38 Mitogen-Activated Protein Kinase(p-38MAPK) in lung homogenate were measured using Western Blot. Pathological changes were evaluated with H&E staining.Results:The mean levels of TNF-α, IL-1β, IL-8 in BALF and MPO in lung homogenate of mice exposed to CS were significantly elevated respectively compared to controls(p<0.05), and the expression of p-ERK and p-38MAPK increased(p<0.05). Chrysin significantly reduced the CS-induced release of TNF-α, IL-1β, IL-8 and MPO respectively comparing with those in mice exposed to CS alone(TNF-α, IL-1β, IL-8: 58.81±1.07 vs 64.41±5.33 pg/ml, 27.46±0.51 vs 34.47±7.55 pg/ml, 129.34±22.08 vs 168.21±16.70 pg/ml)(p<0.05). Meanwhile, chrysin decreased CS-induced ERK, 38MAPK phosphorylation(p<0.05). In addition, chrysin prevented Pathological changes in mice airway induced by CS.Conclusion: Chrysin suppresses airway inflammation induced by cigarette smoke in mice, the phosphorylation of ERK and 38MAPK may be involved in this process. These findings suggest that chrysin has anti-inflammation effect upon CS-induced inflammatory airway diseases. ER -