TY - JOUR T1 - Nontuberculous mycobacterial disease of the lungs in North-West region of Russia JF - European Respiratory Journal JO - Eur Respir J VL - 44 IS - Suppl 58 SP - P2540 AU - Viacheslav Zhuravlev AU - Tatiayna Otten AU - Nataliya Melnikova AU - Ludmila Archakova AU - Pavel Gavrilov AU - Marina Shulgina Y1 - 2014/09/01 UR - http://erj.ersjournals.com/content/44/Suppl_58/P2540.abstract N2 - The incidence of (NTM) as human pathogens is increasing. There are no any statistical data for the incidence of NTM detection. Definitive diagnoses of pulmonary NTM infection are difficult. 450 NTM cultures were isolated from 359 patients with TB suspicion using solid media and/or BACTEC MGIT 960 (2011-13 years). Primary screening of cultures was performed using standard microbiological techniques blood agar culture and ZN stain. For suspected samples species identification using GenoTypeCM/AS (HAIN Lifescience, Germany) were carried out. Following spectra of NTM strains were detected: M.avium (240 strains 53.3%), M.intracellulare 39(8.6%), M.fortuitum 38(8.4%), M.chelonae 38(8.4%), M.abscessus 23( 5.1%), M.peregrinum 19(4.2%), M.lentiflavum 19(4.2%), M.gordonae15(3.3%), M.kansasii 5(1.1%), M.malmoense 5(1.1%), M.xenopi 3(0.6%), M.celatum 3(0.6%), M.scrofulacium 2(0.4% ), M.simiae 1(0.2%). The clinical symptoms of NTM pulmonary disease was variable and nonspecific. The clinical presentation frequently included fever, cough, weight loss, night sweats, hemoptysis, and dyspnea have been revealed only at 102 (28.4%) patients. 38 patients (10.6%) had HIV co infection. Pulmonary infiltrates were the most common (57.4%), disseminated forms (42.6%). The location was unilateral in 53.5% and affection in upper lobes was observed in 79.5% cases.Conclusions:The most common NTM specie in our area is M.avium. Pulmonary disease by NTM has a clinical and radiological presentation similar to tuberculosis. Usage of exact and rapid molecular-genetic method - GenoTypeCM/AS helps to perform accuracy species identification of NTM, it helps physicians to ensure accurate diagnosis and prescribe adequate treatment. ER -