RT Journal Article
SR Electronic
T1 Expression of water and ion channels proteins in acute respiratory distress syndrome from different etiologies
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P3936
VO 44
IS Suppl 58
A1 Ruy Pires-Neto
A1 Ellen Nascimento
A1 Joao Souza
A1 Alfredo Rodrigues
A1 Fabiola Bernardi
A1 Thais Mauad
A1 Luiz Silva
A1 Marisa Dolhnikoff
YR 2014
UL http://erj.ersjournals.com/content/44/Suppl_58/P3936.abstract
AB Introduction: Aquaporins (AQP) and ion channels are membrane proteins that facilitate the rapid movement of water and solute across biological membranes. Diffuse alveolar damage compromises the function of these channels and impairs pulmonary edema resolution. Currently, there is no evidence that these channels are similarly impaired in Acute respiratory distress syndrome (ARDS) of different etiologies. Aim: In this study we quantified the expression of AQP1, Na-K-ATPase and Epithelium Sodium Channel (ENaC) in alveolar septa of patients that died of ARDS from different etiologies. Methods: Using immunohistochemistry and image analysis we analyzed autopsy lung tissue of patients that died with ARDS (n=45) secondary to: Leptospirosis (n=15), Influenza-A H1N1 (n=16) and sepsis (n=14). Two groups of patients were used as controls (n=28): Cardiogenic alveolar edema (n=13) and normal lungs of patients that died of non-pulmonary causes (n=15). Data are shown as positive area/ alveolar epithelial length (µm2/µm). Results: Compared to control groups, ARDS patients showed a lower expression of AQP1 (0.69±0.3 vs 0.53±0.3 µm2/µm; p=0.017) and ENaC (0.91±0.43 vs 0.57±0.33 µm2/µm; p=0.001) and a higher expression of Na-K-ATPase (0.74±0.41 vs 1.26±0.86 µm2/µm; p=0.018). In a subgroup analysis of ARDS patients, there was no difference in proteins expression among ARDS of different etiologies. Conclusion: Water and ions channels are impaired in ARDS patients independently of ARDS etiology.