RT Journal Article
SR Electronic
T1 Surfactant protein expression at various COPD Gold stages
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P3994
VO 44
IS Suppl 58
A1 Sophie Seehase
A1 Henrik Watz
A1 Christian Kugler
A1 Ekkehard Vollmer
A1 Torsten Goldmann
YR 2014
UL http://erj.ersjournals.com/content/44/Suppl_58/P3994.abstract
AB An imbalance of the pulmonary surfactant systems is suggested to be involved in developing chronic obstructive pulmonary disease (COPD). Up to date, there is no detailed knowledge about the expression of surfactant proteins (SP) within the various COPD GOLD stages.Archived HOPE- as well as formalin-fixed paraffin embedded tumor free lung tissues from 37 subjects (GOLD I: 16, GOLD II: 16, GOLD III: 5) were investigated by immunohistochemistry (IHC). From each, single sections were performed and stained for 6 different proteins involved in the surfactant system – SP-A, proSP-B, SP-C, SP-D, haptoglobin, ABCA3. Ten pictures of each IHC stained section were randomly taken avoiding the regions in proximity of the edges of the specimen as well as of larger airways. Quantitative assessment of the IHC staining in the lung tissue was performed. IHC-positive area per total area were correlated with the respective GOLD stages.No differences of proSP-B, SP-D and haptoglobin were detectable in lungs of GOLD stages I to III. The amount of positive area of ABCA3 and SP-A was increased between GOLD I and GOLD III, but not statistically significant. The amount of positive stained SP-C area was significantly increased in lungs GOLD III, compared to GOLD I.Our data revealed that a correlation of surfactant and ABCA3 protein expression in lung tissue of COPD patients is not of clinical relevance. Thus, we will establish a COPD-score considering grade of emphysema and numbers of inflammatory cells, since this appears to be more likely to characterize the relationship of histopathological hallmarks and clinical symptoms in patients suffering from COPD.