TY - JOUR T1 - Change of A549 cells characteristics upon stimulation with BALF of patients with interstitial lung diseases JF - European Respiratory Journal JO - Eur Respir J VL - 44 IS - Suppl 58 SP - P3745 AU - Martina Sterclova AU - Peter Paluch AU - Katharina Heinzelmann AU - Oliver Eickelberg AU - Martina Vasakova Y1 - 2014/09/01 UR - http://erj.ersjournals.com/content/44/Suppl_58/P3745.abstract N2 - Fibrosing interstitial lung diseases were found to have close association with lung cancer. Pathogenetic mechanism remains unclear.The aim of this pilot study was to find out, how do mediators in BALF of patients with fibrosing lung diseases affect immortalized lung epithelial cell line in time.Twelve patients diagnosed with interstitial lung disease underwent HRCT of the chest, pulmonary function tests and bronchoscopy with bronchoalveolar lavage (BAL). Human type II alveolar epithelial cells (cell line A549) were exposed to the BALF of patients in different concentrations for 24 and 48 hours. Unstimulated cells formed the control group. Cells counts, viability and proliferation capacity were compared. Correlations among clinical parameters and A549 characteristics after patients BALF stimulation were sought.Concentration of BALF used for A549 stimulation and duration of exposure of A549 to BALF were found to have significant effect on A549 count, A549 viability and proliferation capacity (p<0,01).24 hours cocultivation: positive correlations among A549 cell counts and viability with HRCT interstitial score-negative correlation between HRCT interstitial score and A549 proliferation capacity (p<0,05)- positive correlation of HRCT alveolar score with A549 proliferation (p<0,05)48 hours cocultivation: positive correlation between HRCT interstitial score and proliferation capacity (p<0,05).Differences beween A549 cell characteristics after 24 and 48 hours may lead us to hypothesis, that prolonged cocultivation of A549 cell line with BALF of patients with fibrotic interstitial lung diseases have probably caused selection of faster proliferating cell subtype. ER -