TY - JOUR T1 - Comparison of tuberculin skin test and in-vitro interferon gamma release tests in diagnosis of tuberculosis in children with household tuberculosis contact JF - European Respiratory Journal JO - Eur Respir J VL - 44 IS - Suppl 58 SP - P3457 AU - Hasan Azem Karabacak AU - Ozge Yilmaz AU - Ibrahim Tuglu AU - Fatma Taneli AU - Suheyla Surucuoglu AU - Esra Toprak Kanik AU - Nuri Ozkutuk AU - Ceyhun Gozukara AU - Mahmud Mustafa Ozkut AU - Ahmet Turkeli AU - Hasan Yuksel Y1 - 2014/09/01 UR - http://erj.ersjournals.com/content/44/Suppl_58/P3457.abstract N2 - Introduction: The aim of this study was to compare nonspecific and specific tuberculosis (TB) antigen IL-18 and IL-27 response of peripheral mononuclear cells in BCG vaccinated children who do and do not develop latent TB after household contact with an adult TB case.Methods: We enrolled 40 children aged 12-60 months who had in-house family member with proven TB to this cross sectional study. We recorded all children's age and gender and tuberculin skin test (TST) result. Blood samples were taken for peripheral blood mononuclear cell culture (PBMC) and QuantiFERON TB test. IL-18 and IL-27 levels were measured in the PBMC supernatant without stimulation and after stimulation with ESAT-6 + CFP-10.Results: Among these children 13 were TST (+) and 27 were TST (-). Quantiferon testing revealed that all 27 TST(-) children were also quantiferon (-) while seven of the TST (+) were quantiferon (+). IL-18 and IL-27 levels in unstimulated PBMC supernatants from TDT (-) Quantiferon (-), TDT (+) Quantiferon (-) and TDT (+) Quantiferon (+) groups were not significantly different (p = 0.956 and p = 0694). Levels of IL-18 and IL-27 in PBMC supernatant after ESAT6+CFP-10 stimulation were significantly different among these three groups (p=0.003 ve p=0.022).Conclusion: Low L-18 and IL-27 response of peripheral blood mononuclear cells levels may play a role in development of TB infection in BCG vaccinated children with household TB contact and this might indicate a specific immune deviation. Moreover, PBMC IL-27 response carries a potential to be used as a diagnostic tool for confirmation of infection in TST (+) BCG vaccinated children. ER -