RT Journal Article
SR Electronic
T1 Viral and bacterial etiologies in acute exacerbation of COPD detected using a multiplex real-time polymerase chain reaction
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P251
VO 44
IS Suppl 58
A1 Kenichiro Shimizu
A1 Yutaka Yoshii
A1 Miyuki Morozumi
A1 Naoko Chiba
A1 Kimiko Ubukata
A1 Hironori Uruga
A1 Shigeo Hanada
A1 Saburo Ito
A1 Naoki Takasaka
A1 Hiromichi Hara
A1 Takanori Numata
A1 Makoto Kawaishi
A1 Jun Araya
A1 Yumi Kaneko
A1 Katsutoshi Nakayama
A1 Kazuyoshi Kuwano
YR 2014
UL http://erj.ersjournals.com/content/44/Suppl_58/P251.abstract
AB Background: Acute exacerbation is a major concern affecting the mortality and morbidity rates associated with COPD. The most common causes of acute exacerbation of COPD (AE_COPD) are respiratory infection by rhinovirus (RV), influenza virus (FLU), Haemophilus influenzae (H.flu), and Streptococcus pneumoniae (S.pn). The causative organisms of AE_COPD identified using a multiplex polymerase chain reaction (MRT-PCR) have rarely been documented. Aims: To evaluate the etiologies in AE_COPD, a rapid reliable process based on MRT-PCR for respiratory samples was used. Methods: From Sep. 2012 to Jan. 2014, we prospectively enrolled AE_COPD patients who satisfied our criteria: an age of 20 years or older, within 7 days of onset, and the informed consent. Nasopharyngeal swabs (NPS) and sputum samples were collected from all the patients, and a MRT-PCR was used to detect 6 types of bacteria and 11 types of respiratory viruses. Bacterial cultures of both specimens were performed. Results: Both bacteria and/or viruses were detected using MRT-PCR in 24 (66.6%) of the 36 patients who satisfied our enrollment criteria. Using acombination of MRT-PCR and culture, the pathogens associated with AE_COPD were identified in 30 (83.3%) of the cases. The most commonmicroorganisms were H.flu, S.pn, RV, and FLU. An elevated CRP level and infection with a microorganism other than H.flu were significantly associated with a need for long-term hospitalization. Conclusions: Our results suggest the usefulness of MRT-PCR for the analysis of NPS and sputum samples to determine the etiology of AE_COPD. Based on the results, we analyzed the association between microorganisms and AE_COPD.