RT Journal Article SR Electronic T1 Allelic polymorphism of IL-4 and IL-10 genes as underlying factor in the production of cytokines and rate of recurrent pulmonary tuberculosis(RPTB) JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP P2690 VO 44 IS Suppl 58 A1 Dmytro Butov A1 Mykhaylo Kuzhko A1 Tatyana Butova A1 Hanna Stepanenko YR 2014 UL http://erj.ersjournals.com/content/44/Suppl_58/P2690.abstract AB Background:To study the allelic polymorphism of IL-4 and IL-10genes in relation to serum levels of cytokines and prevalence of RPTB.Methods:The study comprised 80 individuals in Kharkiv region including 59patientsRPTB(group1) and21healthy donors(group2).Serum levels of cytokines IL-4and IL-10were evaluated by ELISA.Investigations of gene polymorphisms of these cytokines were performed using restriction analysis of the amplification products of specific regions of the genome.Two polymorphic variants were examined:promoter regionC-589T ofIL-4 gene and G-1082A-IL-10.Results:1group-IL-4and IL-10 were 9.55±0.24pg/L and 40.04±0.74pg/L, while in 2group-29.99±1.27pg/L and 50.25±1.26pg/L respectively(p<0.05).Among patients with RPTB the heterozygous genotype was most prevalent;67.79%(N=40)for IL-10and 59.32%35(N=35)for IL-4.The homozygous genotype was accordingly less common:32.21%(N=19)and40.68%(N=24),of which11.86%(N=7)and 15.25%(N=9) of patients had mutation and remaining had normal homozygote genotype,i.e.,20.35%(N=12)and 25.43%(N=15) forIL-10 and IL-4 respectively.In contrast, most of 2group had normal homozygous genotype with 61.9%(N=13)and 71.43%(N=15)with low frequency of mutations;14.30%(N=3)and9.53%(N=2)and heterozygous genotype23.8%(N=5)and19.04%(N=4)for IL-10and IL-4 genes respectively.Conclusion:Compared to healthy controls with RPTB had significantly lower levels of serum IL-4and IL-10.This coincided with greater frequency of heterozygous polymorphism genes of IL-4and IL-10.Further studies are warranted whether higher rate of RPTB has a causal immunogenetic relationship to allelic polymorphism of genes encoding for IL-10andIL-4.