RT Journal Article
SR Electronic
T1 Linezolid penetrates in human pleural cavity: A pilot study using ultra-high pressure liquid chromatography in tandem with mass spectrometry (UPLC-MS/MS)
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P2569
VO 44
IS Suppl 58
A1 Dimosthenis Lykouras
A1 Maria-Eleni Androutsou
A1 Kiriakos Karkoulias
A1 Olga Lagiou
A1 Apostolos Voulgaridis
A1 Maria Tsiamita
A1 Panos Kitrou
A1 Theodore Tselios
A1 Christodoulos Flordellis
A1 John Matsoukas
A1 George Panayiotakopoulos
A1 Kostas Spiropoulos
YR 2014
UL http://erj.ersjournals.com/content/44/Suppl_58/P2569.abstract
AB Introduction: Antibiotics that are used for pneumonia and pleural effusions must have a good bioavailability both in serum and pleural fluid. It is known that linezolid can enter the pleural cavity of rabbits. However, its penetration has never been documented in human pleural cavity. The aim of this study was to develop a new analytical method for the measurement of linezolid in human serum and pleural fluid so as to evaluate the penetration of linezolid in human pleura.Materials and Methods: We developed a novel analytical method to measure linezolid in human serum and pleural fluid using Ultra Performance Liquid Chromatography (UPLC) in tandem with Mass Spectometry (MS/MS). We collected blood samples and the whole pleural fluid that was drained out of the pleural cavity of one patient that was treated with linezolid at usual doses for pneumonia with a pleural effusion. Precipitation of proteins both in serum and pleural fluid was performed using cold AcN. The samples were then analyzed in UPLC-MS/MS.Results: The method was used to measure linezolid levels in pleural fluid of one patient with pneumonia and pleural effusion. The resulting chromatograms showed penetration of linezolid in pleural fluid, while having usual concentrations in human serum.Conclusions: Our new chromatographic method was used for the measurement of linezolid levels in serum and pleural fluid that was collected from one patient, in order to evaluate its efficacy in pleural effusions. To the best of our knowledge, this study clearly shows for the first time that linezolid enters in human pleural cavity and reaches therapeutic concentrations.