TY - JOUR T1 - TNFα receptors on monocytes in peripheral blood mononuclear cells culture in active pulmonary tuberculosis JF - European Respiratory Journal JO - Eur Respir J VL - 44 IS - Suppl 58 SP - P2591 AU - Alina Alshevskaya AU - Fedor Kireev AU - Julia Lopatnikova AU - Zhanna Laushkina AU - Sergey Sennikov Y1 - 2014/09/01 UR - http://erj.ersjournals.com/content/44/Suppl_58/P2591.abstract N2 - Background. Tumor necrosis factor alpha (TNFα) regulate initiation of the adaptive immune response to infection and finally provide protection to the host [Hossain MM, Norazmi MN. Biomed Res Int.2013;2013:179174.]. The main target for mediator in antituberculous immunity are CD14+ cells at which it acts through membrane receptors (TNFRI/II).Objective. To determine changes in TNFRs expression on monocytes in conditions of proinflammatory response induction in patients with active pulmonary tuberculosis (APTB).Methods. Peripheral blood mononuclear cells from 45 APTB patients and 22 healthy donors were cultured overnight in 96-well plates with presence and without LPS. The percent of monocytes expressing TNFRs was determined by flow cytometry using anti-hCD14 FITC (eBioscience, USA) and anti-hTNFRI/II-PE (R&D Systems, USA) antibodies. To count number of receptors per cell Quantibrite PE Beads (BD, USA) were used.Results. Percent of TNFRI+ CD14+ cells has no difference between groups but number of TNFRI per cell in APTB was significantly lower in both unstimulated and LPS-stimulated culture. APTB donors has higher percent of TNFRII+ monocytes with lower number of receptors only in spontaneous culture. LPS increased percent of TNFRII+ monocytes and the number of both type receptors as in APTB so in health.Conclusion. Thus, lower number of TNFRI on CD14+ cells with higher percent of TNFRII+cells in tuberculosis patients compared with healthy individuals and differences between studied group on response to the stimulator are shown various involvement of receptors type I and II controlling different functions of cytokine in pathological process of pulmonary tuberculosis. ER -