@article {KacprzakP2026, author = {Dorota Kacprzak and Joanna Wieczfinska and Ewa Pniewska and Rafal Pawliczak}, title = {Increased oxidative stress and apoptosis susceptibility in peripheral blood mononuclear cells from aspirin sensitive asthmatics in comparison to aspirin tolerant asthmatics and healthy volunteers}, volume = {44}, number = {Suppl 58}, elocation-id = {P2026}, year = {2014}, publisher = {European Respiratory Society}, abstract = {Background: Up to twenty percent of adult asthmatics with nasal polyps are sensitive to aspirin and no unambiguous theory exists which provides a satisfactory explanation for the occurrence of aspirin-induced asthma (AIA) in some asthmatic patients.Aim: The main aim of this study was to recognize possible gene targets involved in the pathogenesis of aspirin-induced asthma in Polish population.Methods: Peripheral blood mononuclear cells (PBMCs) were separated from blood of three groups of subjects - aspirin sensitive asthmatics (ASA), aspirin tolerant asthmatics (ATA) and healthy volunteers (HV) and then stimulated by either lysine aspirin or lysine as a endogenous control. Next, RNA was isolated, transcribed into cDNA and subjected to qPCR (11 ASA, 7 ATA and 15 HV) studies. Simultaneously, protein was extracted from PBMCs and used in further Western Blot analysis (6 ASA, 6 ATA and 7 HV).Results: The analysis of results has shown six genes, which expression at mRNA level was significantly altered between two comprising groups: FOSL1, PARVG, CNPY3, CSF1, MARVELD1, ERAS. At protein level the change in expression of one protein - FOSL1 was confirmed.Conclusion: The ontology analysis shows that a common denominator of most our results is higher oxidative stress and apoptosis susceptibility of AIA than in ATA and healthy controls. Moreover, we observed the lack of peripheral immunogenic processes in the occurrence of hypersensitivity after ingestion of aspirin.Research funded The Polish National Science Centre no 2011/01/N/NZ5/00334.}, issn = {0903-1936}, URL = {https://erj.ersjournals.com/content/44/Suppl_58/P2026}, eprint = {https://erj.ersjournals.com/content}, journal = {European Respiratory Journal} }