RT Journal Article
SR Electronic
T1 Inflammation in COPD patients before and after roflumilast treatment
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P927
VO 44
IS Suppl 58
A1 María Inés Vargas-Rojas
A1 Helena Solleriro-Villavicencio
A1 Alejandra Ramírez-Venegas
A1 Rafael Hernández-Zenteno
A1 Oliver Pérez-Bautista
A1 Abraham Ziga-Martinez
A1 Roger Quintana-Carrillo
A1 Fernando Dueñas-Morales
A1 Rasúl Sansores-Martínez
YR 2014
UL http://erj.ersjournals.com/content/44/Suppl_58/P927.abstract
AB Background.Most of the clinical features of COPD are determined by cytokines, chemokines and growth factors. Roflumilast is a phosphodiesterase type 4 inhibitor, producing an anti-inflammatory effect. It is administered orally as a single daily dose, and it was developed as a treatment to diminish chronic inflammation of COPD patients, although its effect on the level of cytokines and chemokines involved in the polarization of the CD4+ T cells is unknownAim. Determine if Roflumilast is able to reduce the concentration of pro-inflammatory cytokines and chemokines, impacting on the polarization of the CD4+ subpopulationsMaterial and methods. Twenty-four patients with COPD diagnose (GOLD guidelines), candidates to Roflumilast treatment. Peripheral samples were taken, a baseline simple and another 8 weeks after treatment. Through flow cytometry the proportion of CD4+ T cells were defined. Serum cytokines and chemokines were quantified through the Luminex systemResults. All the subjects had a low respiratory function (FEV1 28 (18-36)%) After Roflumilast treatment patients showed a significant reduction of pro-inflammatory cytokines (IL-1b, IL-6 e IL-17) and chemokines (IL-8, eotaxin, IP-10 y MCP-1). Th17 were reduced (pre 5.8±1.5, post 5.4±1.6, p=0.0367) and Treg cells increased (pre 7.7±1.4, post 8.8±1.3, p=0.0000)Conclusions. This study shows that Roflumilast is capable of reducing the concentration of pro-inflammatory cytokines and chemokines in a period as short as 8 weeks. During this time it can be observe decrease of Th17 cells and an increase of Treg cells that suggests a change in the microenvironment that might lead to the development of certain anti-inflammatory mechanisms.