@article {Wagner -StruwingP1138, author = {Carolin Wagner -Struwing and M{\textordfeminine} Angeles Munoz-Lucas and Javier Jareno-Esteban and Concepcion Civera-Tejuca and Jose Angel Maldonado-Sanz and Luis Callol-Sanchez}, title = {Smoking habit and volatile organic compounds in exhaled breath of healthy subjects}, volume = {44}, number = {Suppl 58}, elocation-id = {P1138}, year = {2014}, publisher = {European Respiratory Society}, abstract = {INTRODUCTIONTobacco smoke exposure increases oxidative stress and is related to respiratory diseases. Volatile organic compounds - VOC- (aldehydes and organic acids) in exhaled breath might be biochemical markers of oxidative stress and smoking habit.AIMSTo determine if there exists an association between specific VOC and the smoking habit of healthy subjects.MATERIAL AND METHODSObservational case-control study in healthy volunteers, divided into three groups: smokers, ex -smokers and non-smokers (according to SEPAR criteria) with normal spirometry and no evidence of respiratory disease. Breath samples were collected via a BioVOC container after a residual functional capacity maneuver. We analized six VOC (hexanal, heptanal, octanal, nonanal, propionic acid and nonanoic acid). Biochemical analysis was performed by TD/GC/MS (Markes-Agilent Tech). We used the SPSS{\textregistered} v-15 for Windows for statistical analysis.RESULTSWe included 89 healthy subjects with a mean age of 49.3 years. 42 male, 47 female. 30 were smokers, 24 ex-smokers and 35 non-smokers. The pack-year index was 30.9 (18.6) in smokers and 26.5 (21.6) in ex-smokers.Only nonanal showed statistical differences between the three groups (p 0.04). After dividing into tobacco-exposure (smokers and ex-smokers) and non tobacco exposure group, we also observed a statistical difference (p 0.019).There were no differences after stratification analysis for age and gender (male r 0.05, p 0.753, female r 0.081, p 0.588), andbetween the nonanal concentration and the amount of tobacco smoked.CONCLUSIONSNonanal could be a marker of oxidative stress in smokers and ex {\textendash} smokers.We observed no association with age, gender or amount of tobacco consumption.}, issn = {0903-1936}, URL = {https://erj.ersjournals.com/content/44/Suppl_58/P1138}, eprint = {https://erj.ersjournals.com/content}, journal = {European Respiratory Journal} }