RT Journal Article
SR Electronic
T1 Neutrophil degranulation responses in exacerbators and non-exacerbators of COPD
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P833
VO 44
IS Suppl 58
A1 Arwel Jones
A1 Richard Robinson
A1 Peer Mohammed
A1 Glen Davison
A1 Keir Lewis
YR 2014
UL http://erj.ersjournals.com/content/44/Suppl_58/P833.abstract
AB IntroductionNeutrophilic inflammation, particularly from proteinases such as neutrophil elastase is implicated in the pathogenesis of COPD (Thorax 2006; 61: 250). We compared unstimulated and stimulated production of neutrophil elastase in people suffering >2 exacerbations, needing oral steroids or hospital attendance in the last 12 months and those having no exacerbations.MethodsSubjects: people with COPD (Stage 3-4), prescribed optimal medical therapy and all ex-smokers (eCO<10ppm). Unstimulated and stimulated (bacterial lipopolysaccharide; 840-15, Sigma, UK) neutrophil-specific elastase production was determined with a commercially available enzyme-linked immunosorbent assay (Merck, Germany) from plasma and spontaneous sputum.ResultsView this table:Clinical characteristics and neutrophil-specific elastase concentrationsConclusionFrequent exacerbators have heightened elastase release in response to bacterial stimulation in sputa and a tendency for lower neutrophil-specific elastase concentration at rest in both plasma and sputa compared to non-exacerbators.