TY - JOUR T1 - Feasibility of OSNA in EBUS-TBNA of lung cancer patients JF - European Respiratory Journal JO - Eur Respir J VL - 44 IS - Suppl 58 SP - P3550 AU - Kaid Darwiche AU - Filiz Oezkan AU - Amir Khan AU - Dirk Theegarten AU - Robert Walter AU - Lutz Freitag Y1 - 2014/09/01 UR - http://erj.ersjournals.com/content/44/Suppl_58/P3550.abstract N2 - IntroductionOne Step Nucleic Acid Amplification (OSNA) is a new method in quick detection of sentinel lymph node (SLN) worldwide in breast cancer. OSNA obviates the need for axillary dissection and helps expedite clinical decision making to adjuvant therapy.In lung cancer, regional lymph nodes are assessed before tumor resection. EBUS-TBNA is a well established method for mediastinal lymph node staging providing only small samples instead of surgical biopsies. Our study evaluates OSNA in EBUS-TBNA specimen of lung cancer patients.Methods and MaterialsWe used OSNA with reverse transcription loop-mediated isothermal amplification (RT-LAMP). Using the Lynoamp amplification kit in the RD-100i (Sysmex, Japan), CK19 mRNA concentration was detected. Samples with a CK19 mRNA copy number above 250 copies/µl were regarded as positive, less than 250 copies/ul were defined as negative. We used histopathologic evaluation for standard comparison. 19 EBUS-TBNA lymph node samples were obtained during routine staging. Approximately 3-7 mg of each sample was used for OSNA analysis.ResultsIn 19 patients suspected to have lymph node metastases we found 7 adenocarcinoma, 5 squamous cell carcinoma, 6 small cell lung cancer and 1 negative sample. 15 of 18 patients were detected by OSNA. One negative lymph node was also detected OSNA negative. Overall sensitivity, specificity, positive and negative predictive value and accuracy was 83,3 %, 100%, 100%, 25%, 84,2% respectively. Total procedural time was about 45 minutes for 4 samples.Conclusion OSNA can provide quick and accurate information regarding dignity of EBUS-TBNA specimen. Our preliminary study shows that OSNA may play an important role in diagnosing and staging of lung cancer. ER -