PT  - JOURNAL ARTICLE
AU  - Shuichiro Maruoka
AU  - Yasuhiro Gon
AU  - Sotaro Shikano
AU  - Yoshitaka Shintani
AU  - Daisuke Koyama
AU  - Tadataka Sekiyama
AU  - Hisato Hiranuma
AU  - Toshio Inoue
AU  - Ikuko Takeshita
AU  - Eriko Tsuboi
AU  - Kaori Soda
AU  - Shu Hashimoto
TI  - Exosomal microRNAs in the serum are potential real-time biomarkers for allergic inflammation in the airway of mice
DP  - 2014 Sep 01
TA  - European Respiratory Journal
PG  - P1010
VI  - 44
IP  - Suppl 58
4099  - http://erj.ersjournals.com/content/44/Suppl_58/P1010.short
4100  - http://erj.ersjournals.com/content/44/Suppl_58/P1010.full
SO  - Eur Respir J2014 Sep 01; 44
AB  - Background MicroRNAs (miRNAs) have been reported as useful therapeutic and diagnostic targets for patients with asthma. Even ribonucleases (RNase) are present throughout the human body, miRNAs is stably exist in body fluids because they are contained in exosomes. However, little is known regarding which exosomal miRNAs in the serum are suitable for the real-time monitoring of allergic inflammation in the airway. We therefore investigated miRNA expression profiles of serum exosomes and lung tissues isolated from house dust mite (HDM)-exposed mice. Methods We sensitized and challenged mice with HDM or saline as control on day1, 8 and 15. On day 18, we collected lung tissues and serum from HDM- or saline- exposed mice. Total RNAs were extracted from lung tissues using total RNA isolation kit and serum exosomes using exosomal RNA purification kit. Then we performed miRNA array analysis to identify exosomal miRNAs in the serum, which might be involved in ongoing allergic airway inflammation in the airway of mice. Data analysis was performed using GeneSpring software. Gene network analysis was performed using Ingenuity Pathways Analysis. We also validated gene expression of candidate miRNAs using real-time PCR. Results We identified 100 HDM-inducible miRNAs in serum exosomes and 175 miRNAs in lung tissues. 5 up-regulated miRNAs or 5 down-regulated miRNAs were found to be common in both serum exosomes and lung tissues from HDM-sensitized mice. Conclusions Our data suggest that exosomal miRNAs in the serum we identified might be new diagnostic biomarkers or therapeutic targets for allergic inflammation in the airway.