RT Journal Article
SR Electronic
T1 Late-breaking abstract: The effects and mechanism of adiponectin on inhibiting pulmonary fibrosis
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P733
VO 44
IS Suppl 58
A1 Rong Yao
A1 Yu Cao
A1 Ya-rong He
A1 Zhi Zeng
A1 Zong-an Liang
YR 2014
UL http://erj.ersjournals.com/content/44/Suppl_58/P733.abstract
AB Introduction Pulmonary fibrosis is a common chronic lung disease which calls for more effective therapies. Accumulating evidence has suggested that adiponectin (APN) maybe a promising therapeutic option in fibrotic diseases.Aims and objectives Here we aim to evaluate whether exogenous globular form of APN could protect against pulmonary fibrosis in paraquat (PQ) -treated mice and human lung fibroblasts.Methods BalB/C mice were divided into four groups (Control group, PQ intoxication group, PQ + low dose APN group and PQ + high dose APN group) and sacrificed on day 3, 7, 14 and 21. Lung tissue and blood were collected and analyzed histologically and biochemically. Human lung fibroblasts WI-38 were divided into four groups (Control group, PQ intoxication group, APN group and AdipoR1 siRNA group) and collected for measurements at 24, 48 and 72 h after PQ stimulation.Results PQ significantly increased the lung fibrosis scores, expression of MMP-9 and TIMP-1 in blood, as well as TGF-β1 and á-SMA in lung tissue. Also, PQ significantly decreased cell viability and up-regulated the expression of á-SMA, Collagen type III in lung fibroblasts. All of these changes were remarkably inhibited by APN treatment in a dose-dependent manner. There are APN, AdipoR1 and AdipoR2 expressed by human WI-38 lung fibroblasts.The protective effects mediated by APN on PQ treated lung fibroblasts were abrogated by transfected with AdipoR1 siRNA.Conclusions Overall, these studies indicated some protective effects of APN on PQ induced pulmonary fibrosis in a dose-dependent manner. There are APN and functional AdipoR1 expressed by human WI-38 lung fibroblasts, suggesting a potential application prospect of APN for lung fibrosis.