TY - JOUR T1 - Forced oscillation technique for evaluation of <em>ex vivo</em> perfused pig lungs JF - European Respiratory Journal JO - Eur Respir J VL - 44 IS - Suppl 58 SP - P261 AU - Marie-Hélène Schmidt AU - Sam Bayat AU - Zoltan Hantos AU - Stéphane Gennai AU - Maxime Maignan AU - Raphaël Briot Y1 - 2014/09/01 UR - http://erj.ersjournals.com/content/44/Suppl_58/P261.abstract N2 - Ex vivo lung perfusion (EVLP) is an isolated lung preparation clinically used to evaluate, with few hemodynamic or gazometric parameters, lung grafts initially rejected for transplantation1. Forced Oscillation Technique (FOT) is aimed to measure respiratory mechanics2. The objective of our work was to test the association between FOT parameters and the severity of ischemia reperfusion injury during an EVLP procedure.Methods:Isolated piglungs (n=5) were surgically collected at 4°C, then gradually warmed, perfused and ventilated, according to commonly accepted EVLP methods. After a 45 min steady state at full ventilation and perfusion, blood gases (PaO2/FiO2) and mean pulmonary artery pressure (mPAP) were measured. In the same time, FOT measurements were performed and estimated the airway resistance and inertance, and the tissue parameters: resistance (G) and elastance (H). Correlations were tested between these FOT parameters, PaO2/FiO2 and mPAP.Results:Airway inertance correlated with mPAP (p&lt;0.05; R2=0.798), while airway resistance correlated with Pa02/FiO2 (p&lt;0.05; R2=0.812).The tissue resistance/elastance ratio (G/H) showed a significant positive correlation with both mPAP (p=0.04; R2=0.805) and PaO2/FiO2 (p=0.03; R2=0.812).Discussion:FOT measurements seem to correlate with parameters clinically used for lung grafts evaluation with EVLP method. This is a noninvasive technique allowing simple and repeated measurements. The analysis model estimates separately airways and pulmonary parenchyma. However further investigations are needed, to validate FOT in EVLP before human lung transplantation.References1) Cypel, M.; 2011; N Engl J Med; 364; 15: 1431-402) Oostveen, E.; 2003; Eur Respir J; 22: 1026-1041. ER -