TY - JOUR T1 - Autoreactivity in patients with combined COPD and asthma phenotype JF - European Respiratory Journal JO - Eur Respir J VL - 44 IS - Suppl 58 SP - 4840 AU - Anna Konishcheva AU - Valentina Gervazieva AU - Tatyana Ospelnikova Y1 - 2014/09/01 UR - http://erj.ersjournals.com/content/44/Suppl_58/4840.abstract N2 - Chronic obstructive pulmonary disease (COPD) and severe bronchial asthma (BA) often share inflammatory features, including autoimmunity. We aimed to reveal if the assessment of IgG4/IgE auto Abs to lung tissue Ags in cohorts of patients with COPD and BA could be applied in disease monitoring. Production of IL-4,IL-10,IL-17,TGF-β1 by PBMCs in blood and induced sputum samples from 86 patients with combined COPD and BA (GOLD III/IV) with different BMI, 34 atopic BA and 20 controls. IgG4-and IgE-Abs to human lung elastin, collagen III and myosin were detected by quantitative modified ELISA. 69% of patients with combined COPD/BA showed elevated levels of IgE-Abs to myosin versus atopic BA and oppositely had lower rate (21%) and levels of IgG4-Abs to myosin, keratin and collagen III, as compared with BA (p<0,05). In controls IgG4-autoAbs were not been detected. The group of COPD/BA patients, positive for IgG4-autoAbs, had enhanced production of IL-8 (p<0,001). IgG4-positivity was associated with enhanced sputum levels of IL-4 and a profibrotic cytokine-TGF-β1 only in atopic BA. 70% of COPD/BA patients had emphysema and showed predominance of IgE- vs. IgG4-Abs to collagen III and myosin. 60% of patients had concomitant obesity (BMI≤30 kg\m2), which was independently associated with enhanced production of IL-17 and higher levels of antielastin IgG4-Abs (r=0,87).The level of serum leptin increased in BA vs. COPD especially among patients, positive for anti-elastin IgG4-Abs (p < 0,001). Our results raises the possibility that the detection of IgG4/IgE Abs to lung elastin, collagen and myosin could be considered for further trials in monitoring of COPD patients, especially with combined atopic BA and obese phenotypes. ER -