PT  - JOURNAL ARTICLE
AU  - Carlos Rio
AU  - Andreas Jahn
AU  - Pau Marti
AU  - Amanda Iglesias
AU  - Laura Fueyo
AU  - Orlando Gigirey
AU  - Juan Antonio Torrecilla
AU  - Angel Carvajal
AU  - Catalina Balaguer
AU  - Aina Noguera
AU  - Alejandro Peralta
AU  - Luis Ortiz
AU  - Ernest Sala-Llinas
TI  - Functional and molecular disparities between mesenchymal stem cells from various pulmonary disorders
DP  - 2014 Sep 01
TA  - European Respiratory Journal
PG  - 1420
VI  - 44
IP  - Suppl 58
4099  - http://erj.ersjournals.com/content/44/Suppl_58/1420.short
4100  - http://erj.ersjournals.com/content/44/Suppl_58/1420.full
SO  - Eur Respir J2014 Sep 01; 44
AB  - Introduction: Mesenchymal Stem Cells (MSCs) have been shown to contribute to pulmonary repair and regeneration in lung injury models. However, it is still unknown whether MSCs from patients with chronic diseases have the same regenerative / reparative capacity than MSCs from healthy subjects (H).Aims: To study the functional and molecular response of bone marrow (BM)-MSCs and adipose derived (AD)-MSCs from patients with chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis (IPF) and H.Methods: We have evaluated: 1) the cellular response of MSCs to VEGFs, PDFGs, tobacco; and, 2) the expression level of some of the associated growth factor receptors (GFR). MSCs were isolated from sternal aspirated BM samples and subcutaneous fat from subjects undergoing thoracic surgery. MSC functional response was real time monitored with the xCELLigence system. The amount of GFR was analyzed by RT-PCR and with LI-COR's Odyssey system. Tyrosine phosphorylation was checked by Western Blot.Results: 1) MSCs from different origins respond to VEGF121, VEGF165, PDGFAA and PDFGBB indistinctive ways; 2) PDGFBB elicits the greatest response in tyrosine phosphorylation; 3) BM-COPD cells and ADSC-IPF cells have a significantly higher PDGFR beta expression than cells from H; and, 4) There seems to be a gradient response to tobacco stimulation, being MSC-COPD cells more sensitive than cells from other origins.Conclusions: MSC-COPD and ADSC-IPF exhibit molecular and functional differences. Additional evaluation of these cells is needed to elucidate their potential involvement in the pathogenesis of these diseases.Supported by PI10-00983, PI12-01152, SEPAR 2011 and 2013.