RT Journal Article
SR Electronic
T1 Novel antiviral properties of vitamin D in cystic fibrosis airway epithelial cells
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 3449
VO 44
IS Suppl 58
A1 Aline Schoegler
A1 Brigitte S. Kopf
A1 Ricardo J. Muster
A1 Carmen Casaulta
A1 Andreas Jung
A1 Alexander Moeller
A1 Thomas Geiser
A1 Marco P. Alves
A1 Nicolas Regamey
YR 2014
UL http://erj.ersjournals.com/content/44/Suppl_58/3449.abstract
AB Introduction. CF patients suffer from pulmonary exacerbations induced by virus infections, most frequently triggered by rhinoviruses (RVs). Vitamin D deficiency is a widespread feature of CF patients. Recent evidences suggest that Vitamin D has important immunomodulatory activities in the defense towards pathogens. We hypothesize that Vitamin D has antiviral properties in primary CF airway epithelial cells.Methods. Primary bronchial epithelial cells (BECs) from CF children were pretreated with different concentrations of Vitamin D (10-8 to 10-6 M) and infected with the major group virus RV16. Viral RNA and RV16 surface receptor intercellular adhesion molecule-1 (ICAM-1), pattern recognition receptors (PRRs) involved in detection of RV16, interferon (IFN)-λ2/3, viperin and the antimicrobial peptides cathelicidin and β-defensin expression was assessed by RT-qPCR. Levels of pro-inflammatory cytokines were determined by ELISA. Cell death was measured by Flow cytometry.Results. Vitamin D reduced RV16 load in a dose-dependent manner in CF BECs (10-6M: p<0.01). The expression of ICAM-1, PRRs, IFN-λ2/3 and viperin was unaffected by Vitamin D pretreatment in CF BECs. Similarly, Vitamin D did not modify the expression of β-defensin. However, Vitamin D induced a dose-dependent tendency towards increased cathelicidin expression in CF BECs (p=0.07). Vitamin D did not alter the pro-inflammatory cytokine production.Conclusion. Vitamin D lowers RV16 replication in primary paediatric CF BECs possibly through the induction of the antimicrobial peptide cathelicidin. Our in vitro study may point to the importance of an adequate control of the Vitamin D status in CF patients to prevent virus-induced exacerbations.