RT Journal Article
SR Electronic
T1 Expression of the B cell differentiation factor BAFF and chemokine CXCL13 in a murine model of human respiratory syncytial virus infection
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P3880
VO 42
IS Suppl 57
A1 Wael Alturaiki
A1 Amanda McFarlane
A1 Paul Fitch
A1 Joseph Slupsky
A1 Paul McNamara
A1 Jürgen Schwarze
A1 Brian Flanagan
YR 2013
UL http://erj.ersjournals.com/content/42/Suppl_57/P3880.abstract
AB BACKGROUND AND AIMS:Production of antibody in the lungs is an essential defense mechanism against respiratory pathogens. However, little is known about the mechanisms of local B cell activation in airway mucosa. Previously, we have demonstrated expression of the B cell differentiation factor, BAFF in human RSV infection (McNamara et al 2013 Thorax).To better understand this process we examined BAFF expression in a murine model of RSV infection and measured expression of the chemokines CXCL13, CCl19 and CCl21 which could influence lymphocyte recruitment.METHODS:We measured BAFF, CXCL13, CCL19 and CCL21 expression in homogenised lung tissue from control mice at day 0 and mice challenged with RSV (A2 strain) or control UV-treated RSV at days 1,2,4 or 7 post infection (n=3) by ELISA. Cytokine mRNA and RSV N gene expression were measured by Taqman PCR.RESULTS:BAFF was elevated significantly post RSV infection at day1 (mean1092pg/ml, P=0.07), day2 (1157pg/ml, P=0.01) and day 7(2941 pg/ml, P=0.0001) in comparison to UV treated RSV control on day1 (421pg/ml), day2 (370 pg/ml) and day7 (393pg/ml). BAFF mRNA expression was similarly increased on day 1 (fold increase 2, P=0.03) and day 7(1.7, P=0.02).CXCL13 protein was increased post RSV infection at day 1 (mean762pg/ml, P=0.01), day2 (612pg/ml, P=0.02), and day 7 (388pg/ml, P=0.007) in comparison to control day 1 (374pg/ml), day2 (278pg/ml) and day7 (249pg/ml). CCL19 or CCL21 levels were not increased.CONCLUSION:RSV infection results in up-regulated BAFF and CXCl13 expression, consistent with a role for CXCL13 in recruiting B cells and BAFF in promoting airway B cell differentiation.