PT - JOURNAL ARTICLE AU - Takeo Ishikawa AU - Katsutoshi Nakayama AU - Jiro Mikami AU - Saburo Ito AU - Naoki Takasaka AU - Yoko Yumino AU - Satoko Fujii AU - Chikako Tsurushige AU - Jun Kojima AU - Takanori Numata AU - Hiromichi Hara AU - Makoto Kawaishi AU - Jun Araya AU - Kazuyoshi Kuwano TI - The involvement of thrombospondin-1 in COPD pathogenesis through IL-8 production DP - 2013 Sep 01 TA - European Respiratory Journal PG - P620 VI - 42 IP - Suppl 57 4099 - http://erj.ersjournals.com/content/42/Suppl_57/P620.short 4100 - http://erj.ersjournals.com/content/42/Suppl_57/P620.full SO - Eur Respir J2013 Sep 01; 42 AB - Rationale: The airflow limitation of COPD is related to neutrophilic inflammation and subepithelial fibrosis in the small airways. Thrombospondin-1(TSP-1)is reported to be a key molecule downstream of MyD88/TLRs, which is associated with smoking-related airway inflammation. TSP-1 is also known as a mediator of fibrosis in mouse airway epithelial cells. However, the role of TSP-1 in COPD pathogenesis is not yet fully evaluated. We examined the correlation between TSP-1 expression and lung function, and involvement of TSP-1 in COPD pathogenesis in patients with smoking history.Methods: Human bronchial epithelial cells (HBECs) were isolated from surgical lung specimens and cultured. 48h later, supernatants were recovered. TSP-1 concentrations in supernatants were evaluated by ELISA. The correlation between TSP-1 and pulmonary function was analyzed with linear regression analysis. IL-8 production induced by TSP-1 in HBECs was analyzed by ELISA.Results: Surgical specimens were classified as non-smokers, healthy smokers, or COPD patients. In COPD patients, TSP-1 concentrations were found to be higher than those in non-smokers and healthy smokers. In a correlation analysis between TSP-1 and pulmonary function, TSP-1 had an inverse correlation with %FEV1 and FEV1/FVC. IL-8 concentrations rose after stimulation by TSP-1 in a dose-dependent manner.Conclusions: TSP-1 expression in lung specimen from subjects with smoking history appears to be associated with obstructive ventilatory impairment. It was suggested that IL-8 expression by TSP-1 could be involved in pathogenesis of COPD.