RT Journal Article SR Electronic T1 Diagnosis of pathological changes in bronchial tree using focal fluorescence spectroscopy JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP P2904 VO 42 IS Suppl 57 A1 Nina Poliakova A1 Olga Cheremisina A1 Natalia Bulgakova YR 2013 UL http://erj.ersjournals.com/content/42/Suppl_57/P2904.abstract AB Background: Early diagnosis of recurrence of central lung cancer is a major problem in oncology.Objectives: Evaluate possibilities of employing Focal Fluorescence Spectroscopy (FFS) method in a diagnosis of pathological changes in bronchial tree.Methods: The study includes the follow-up results of 68 patients with non-small cell lung cancer who have received a combination therapy.During the follow-up period there has been provided a traditional bronchoscopy, which was supplemented by FFS of bronchial mucosa with a measurement of autofluorescence (AF). AF spectra were measured in the mucosa of a bronchus resection stump and intact bronchus in the symmetrical site of the contralateral lung.There has been analyzed two spectral features: the intensity at the maximum range of the AF and magnitude of spectral fluorescence diagnostic parameter Df. After measuring the AF, there has been taken biopsy specimens of bronchial mucosa for a morphological study.Results: Laser-excited autofluorescence in the green region of the spectrum of normal bronchial epithelium intensely fluoresces with a maximum wavelength near 580 nm. In the case of an acute inflammation of mucosa there has been a significant decline of the maximum intensity and increase in the parameter Df. In the areas of malignancy, there has been a rapid decline (up to 10-fold) of the autofluorescence intensity along with rising of the spectral diagnostic parameter Df.Conclusions: Analysis of the spectra of laser-induced autofluorescence and an estimate of the spectral diagnostic parameter Df has revealed significant differences in the spectra of normal bronchial epithelium versus acute inflammation or malignant lesions.