RT Journal Article SR Electronic T1 Circulating microparticles levels and vascular function in a mouse model of combined intermittent hypoxia and high-fat diet JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP P2555 VO 42 IS Suppl 57 A1 Wojciech Trzepizur A1 Gaceb Abderahim A1 Claire Arnaud A1 Christophe Ribuot A1 Patrick Levy A1 Ramaroson Andriantsitohaina A1 Maria Carmen Martinez A1 Frederic Gagnadoux YR 2013 UL http://erj.ersjournals.com/content/42/Suppl_57/P2555.abstract AB There is a clinical co-incidence of the metabolic syndrome and the obstructive sleep apnea syndrome (OSAS), and both are associated with cardio-vascular diseases. Microparticles (MPs) are submicron membrane vesicles, released into the extracellular space following to cell activation, implicated in the vascular damage associated with both syndromes.The aim of this study was to evaluate the specific effects of intermittent hypoxia, obesity and the combination of both conditions on MPs levels and endothelial function.Fifty two mice were divided in four groups: control group, high-fat diet (HFD) (42% calories from fat for 8 weeks) receiving group, intermittent hypoxia (IH) group (for 2 weeks,8hours of IH/day, 1 stimulus by 30-60s,) and a group receiving both HFD and IHWe showed that HFD was able to induce early-onset obesity as reflecting by the increase of adipose tissue weight, dyslipidemia and a decrease in the endothelium-dependent relaxation evoked by acetylcholine. Exposure to IH was responsible for an increase in the rate of leukocyte MPs, without changes in endothelium-dependent relaxation. Animals submitted to the combination of both treatments displayed a similar profile of MPs like to only IH treatment and showed an insulin resistance as accessed by HOMA index. In addition, IH prevented endothelial dysfunction induced by HFD alone.This study suggests a specific role of IH in the increased leukocyte derived MPs levels observed in OSAS and in the insulin resistance induced in the HFD model. Interestingly, IH for 2 weeks might be considered as a preconditionning mechanism inducing protection of the vascular function in HFD models.