RT Journal Article
SR Electronic
T1 Comparison of expression of napsin A and surfactant protein D in bleomycin-induced pulmonary fibrosis mouse model
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P2348
VO 42
IS Suppl 57
A1 Takuya Samukawa
A1 Tsutomu Hamada
A1 Tomohiro Kumamoto
A1 Go Tsukuya
A1 Shingo Kubota
A1 Masaki Watanabe
A1 Hiromasa Inoue
YR 2013
UL http://erj.ersjournals.com/content/42/Suppl_57/P2348.abstract
AB Introduction:We have shown elevation of serum napsin A in patients with idiopathic pulmonary fibrosis (IPF), and suggest that napsin A may be a candidate biomarker for IPF. However, napsin A has not been confirmed as a marker for bleomycin-induced pulmonary fibrosis model.Objective: To confirm expression of napsin A in bleomycin-induced pulmonary fibrosis mouse model compared with that of surfactant protein D (SP-D).Methods: Pulmonary fibrosis was induced in C57BL/6J mice by intratracheal injection of 3.0 mg/kg of bleomycin. Five mice underwent bronchoalveolar lavage (BAL) and were sacrificed on 5 individual days, including before the injection (Day 0) and at 2, 7, 14, and 21 days after. The levels of napsin A and SP-D in BAL fluids and serum were measured by ELISA (Mouse Napsin-A kit, CUSABIO, China; Rat/Mouse SP-D kit YAMASA EIA, Yamasa, Japan). Lung tissues were used for immunohistochemistry (IHC) analysis.Result: Napsin A levels in BAL fluids were 119.01 ± 23.63 pg/ml for Day 0, 561.86 ± 178.53 pg/ml for Day 7, and 571.70 ± 279.18 pg/ml for Day 21. Napsin A levels in BAL fluids in bleomycin-induced model were significantly increased after Day 7 and were still detected on Day 21 (p<0.001). However serum napsin A levels showed no significant elevation. SP-D was significantly elevated in BALF fluids and serum (p<0.001). IHC analysis of bleomycin-induced model revealed elevated napsin A and SP-D in lung epithelial cells.Conclusions: The present study demonstrated napsin A as a marker for bleomycin-induced pulmonary-fibrosis model in BAL fluids and lung tissues.