PT - JOURNAL ARTICLE AU - Walter Zin AU - Maria Diana Gomes AU - Giovanna Carvalho AU - Andressa Araujo AU - Natalia Casquilho AU - Manuella Lanzetti AU - Samuel Valença AU - Jose Henrique Leal-Cardoso TI - Can 2,2′-azobis (2-methylpropionamidine) dihydrochloride (AAPH) impair rat lung homeostasis? DP - 2013 Sep 01 TA - European Respiratory Journal PG - P3986 VI - 42 IP - Suppl 57 4099 - http://erj.ersjournals.com/content/42/Suppl_57/P3986.short 4100 - http://erj.ersjournals.com/content/42/Suppl_57/P3986.full SO - Eur Respir J2013 Sep 01; 42 AB - Rationale: Several studies reported that respiratory diseases may be associated with increased free radicals (FR) production. AAPH is a FR-generating compound.Aim: Investigate whether AAPH can impair lung function and alter biochemical markers.Methods: This study was approved by the Ethics Committee on Animal Use (CEUA/CCS/UFRJ) under code IBCCF128. Male Wistar rats received one intranasal instillation of saline solution (714 μL/kg, SS group, n=8) or different amounts of AAPH (25, 50, 100 and 200 mg/kg in saline, 714 μL/kg, AAPH groups, n=28). 72 h after the instillation pulmonary mechanics was determined and lung homogenates were biochemically assayed for antioxidant status. One-way ANOVA followed by Holm-Sidak test was used. The significance level was 5%.Results: Central airway resistive pressure was significantly higher in AAPH100 and AAPH200 [0.739±0.251 and 0.916±0.455 cmH2O, respectively (mean ± SD)] than in SS (0.378±0.120 cmH2O). Static elastance was significantly larger in AAPH200 (2.719±0.618 cmH2O/mL) than in SS (2.102±0.312 cmH2O/mL). Superoxide dismutase activity in AAPH200 was smaller than in AAPH25 (2.451±0.495 and 6.443±1.060 U/mg protein, respectively) and both were significantly different from those in SS, AAPH50 and AAPH100 (5.207±1.024, 5.301±1.221, and 4.824±0.594 U/mg protein, respectively). Catalase activity in AAPH200 (17.319±3.696 U/mg protein) was significantly higher than in SS, AAPH25, AAPH50, and AAPH100 (7.626±1.586, 8.088±1.615, 10.333±2.307, and 9.478±2.098 U/mg protein, respectively).Conclusion: Dose-dependently, AAPH could impair pulmonary mechanics and induce oxidant-antioxidant imbalance.Sources of research support: CNPQ, FAPERJ, CAPES, FUNCAP.