PT - JOURNAL ARTICLE AU - M.A. Abdullaeva AU - E. Kh. Anaev AU - N.B. Karmen AU - L.V. Tokareva AU - T.N. Anokhina TI - Various biomarkers of oxidative stress in patients with uncontrolled asthma DP - 2013 Sep 01 TA - European Respiratory Journal PG - P2012 VI - 42 IP - Suppl 57 4099 - http://erj.ersjournals.com/content/42/Suppl_57/P2012.short 4100 - http://erj.ersjournals.com/content/42/Suppl_57/P2012.full SO - Eur Respir J2013 Sep 01; 42 AB - The aim of this study was to measure the dynamics of various biomarkers of oxidative stress in patients with uncontrolled asthma after 2 weeks of standard treatment.Methods: 33 patients with moderate-to-severe uncontrolled asthma were recruited in the study (20 male, age 48.6±8.4 yrs, ACT<20 scores). Besides clinical data and functional parameters, we assessed the structural-functional parameters of erythrocyte membranes, the content of the products of lipid peroxidation - malondialdehyde (MDA), Schiff bases, diene conjugates (DC) and activity of antioxidant enzymes - superoxide dismutase (SOD) and catalase, tocopherol in the membranes of erythrocytes and plasma.Results: There were significantly improved clinical signs of asthma and pulmonary ventilation parameters after treatment. The intensity of cough, dyspnea and relief medication decreased (p<0.05); FVC, FEV1 and FEV1/FVC ratio increased by 16%, 60% and 38% respectively (p<0.05). We found significant improvements of the structural-functional properties of the erythrocyte membranes: decreasing of microviscosity and polarity annular lipids, increasing of the lipid layer polarity. There was found decreasing of lipid peroxidation activity in erythrocyte membranes: the concentration of DC, MDA and Schiff bases significant decreased in 1.5; 1.8 and 1.4 times respectively (p<0.05). The activity of antioxidant enzymes such as catalase and SOD increased by 24% and 45% (p<0.05). Also the concentration of tocopherol increased in 1.5 times in comparison with the initial level (p<0.02).Conclusion: The dynamics of various biomarkers of oxidative stress in patients with uncontrolled asthma is a reliable method for assessing and monitoring airway inflammation.