RT Journal Article
SR Electronic
T1 Cultured satellite cells as a model for the study of muscle dysfunction in COPD
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P1828
VO 42
IS Suppl 57
A1 Pascal Pomiès
A1 Julie Rodriguez
A1 Sami Sedraoui
A1 Fares Gouzi
A1 Gilles Carnac
A1 Jacques Mercier
A1 Christian Préfaut
A1 Maurice Hayot
YR 2013
UL http://erj.ersjournals.com/content/42/Suppl_57/P1828.abstract
AB The mechanisms leading to muscle dysfunction in COPD have not been elucidated. One hypothesis is the exhausted muscle regenerative capacity of satellite cells, which would compromise muscle mass maintenance, but the capacity of satellite cells to proliferate and differentiate properly in COPD is unknown.The objective was to compare the characteristics of satellite cells derived from COPD patients and healthy subjects, in terms of proliferative and differentiation capacities, morphological phenotype, and oxidative stress status. Therefore, we purified and cultivated satellite cells from frozen quadriceps biopsies of 8 COPD patients and 8 healthy controls. We examined proliferation parameters, the myogenic fusion index, myotube diameter, cell susceptibility to oxidative stress, antioxidant enzyme expression, protein carbonylation, and lipid peroxidation in cultured myoblasts and/or myotubes. The myogenic fusion index was similar in myotubes from controls and COPD patients. Myotube diameter was significantly smaller in COPD patients. Significant correlations were observed between the cultured myotube diameter and fiber cross sectional area as well as muscle voluntary contraction. Furthermore, myoblasts from COPD patients were significantly more susceptible to H2O2-induced oxidative stress than those from control subjects, and oxidative stress damage, like protein carbonylation and lipid peroxidation, was higher in COPD myoblasts and/or myotubes.In conclusion, cultured satellite cells from COPD patients display characteristics of atrophy and oxidative stress similar to those in in vivo COPD limb muscles, suggesting that we have developed a cellular model for the study of muscle dysfunction in COPD.