TY - JOUR T1 - Time course effects of exercise training on pulmonary injury induced by exposure to cigarette smoke in mice JF - European Respiratory Journal JO - Eur Respir J VL - 42 IS - Suppl 57 SP - P1067 AU - Alessandra C. Toledo-Arruda AU - Flávia Guarnier AU - Camila L. Suehiro AU - Francine Almeida AU - Clarice Olivo AU - Fernanda Lopes AU - Rodolfo Vieira AU - José Carlos Silva Camargo-Filho AU - Rubens Cecchini AU - Chin Lin AU - Mílton A. Martins Y1 - 2013/09/01 UR - http://erj.ersjournals.com/content/42/Suppl_57/P1067.abstract N2 - Rationale: Experimental study recently developed by our group showed that the regular physical training attenuated the pulmonary injury in an experimental model of chronic exposure to cigarette smoke (CS). Objective: The goal of this study was to evaluate the time course effects of the mechanisms related to the exercise protection. Methods: Male C57BL/6 mice were divided into four groups: control, exercise, smoke and smoke+exercise. Exposure to CS and treadmill training were carried out: 5 days/week for 4, 8 and 12 weeks. We evaluated lung mechanics by using a FlexiVent ventilator (Scireq, Montreal, Canada); the number of total and differentials cells in bronchoalveolar lavage fluid (BALF); mean linear intercept (LM); TNFα, IL-1β, IL6, IL10, TBARS, antioxidant enzymes (SOD, Gpx and TRAP) in the lung tissue. Results: Exercise protected mice exposed to CS from the: reduction on tissue damping and tissue elastance (p<0.01) after 12 weeks; increase in total inflammatory cells in BALF preferably due to recruitment of neutrophils and lymphocytes after 8 weeks and lymphocytes and macrophages after 12 weeks (p<0.001) and increase in LM after 12 weeks. The protection conferred by exercise in mice exposed to CS was induced by an increase in IL6, IL10 and antioxidants enzymes (SOD, GSH/GSSG, TRAP) and a decrease in TNFα and TBARS levels. Conclusion: Exercise protection in mice exposed to CS is more pronounced after 12 weeks and the mechanisms involved include anti-inflammatory mediators and antioxidants enzymes that have an important role in COPD development.. ER -