PT  - JOURNAL ARTICLE
AU  - Priscila Marcondes
AU  - Regiane Albertini
AU  - Ana Paula Ligeiro de Oliveira
AU  - Rodolfo Vieira
AU  - Flavio Aimbire
TI  - Low-level laser therapy effect on the bronchial hyperresponsiveness and the chronic allergy airway inflammation is driven to RhoA signaling and STAT-6
DP  - 2013 Sep 01
TA  - European Respiratory Journal
PG  - P1573
VI  - 42
IP  - Suppl 57
4099  - http://erj.ersjournals.com/content/42/Suppl_57/P1573.short
4100  - http://erj.ersjournals.com/content/42/Suppl_57/P1573.full
SO  - Eur Respir J2013 Sep 01; 42
AB  - Low-level laser therapy (LLLT) controls the bronchial hyperresponsiveness (BHR) associated with increased RhoA mRNA expression as well as the generation of pro-inflammatory mediators associated with NF-kB signaling in acute lung inflammation. Thus, in the current study, we aimed to explore if LLLT reduce both the BHR and the generation of inflammatory mediators in chronic allergic asthma. Wistar rats were divided in experimental groups for studies of bronchial reactivity and lung inflammation after antigen challenge. The BHR was measured through of isolated organ system and construction of dose-response curves to methacholine. The lung inflammation was measured by the following markers: inflammatory cells, adhesion molecule, mucus production, collagen synthesis and cytokines. To confirm the participation of RhoA in BHR, the animals were pretreated with a RhoA inhibitor, Y-27632, 1 h before the antigenic challenge. LLLT was carried out by a 660 nm diode laser with a dose of 5.4 J during 3 minutes and two irradiation protocols were used. The reduction of BHR post LLLT coincides with decreasing of RhoA expression in bronchial muscle as well as the reduction of eosinophils. Laser effect was also evidenced in E-selectin expression and Th2 cytokines and eotaxin levels. The signal transducer and activator of transduction-6 (STAT6) concentration in lung from challenged rats was also reduced diminished after laser treatment; otherwise, LLLT does not work on IgE in serum. Therefore, our results demonstrated that LLLT reduces the BHR via RhoA and the chronic allergic airway inflammation via STAT6.