TY - JOUR T1 - Analysis of ciliary beating in reconstituted bronchial epithelium from chronic airway diseases JF - European Respiratory Journal JO - Eur Respir J VL - 42 IS - Suppl 57 SP - P3132 AU - Mustapha-Kamel Khelloufi AU - Delphine Gras AU - Céline Garulli AU - Annie Viallat AU - Pascal Chanez Y1 - 2013/09/01 UR - http://erj.ersjournals.com/content/42/Suppl_57/P3132.abstract N2 - Background. Hypersecretion of mucus is frequent in chronic airway diseases and a burden for patients but mechanisms are largely unknown. Aims. We used an original model of in vitro bronchial epithelium reconstruction in air/liquid interface to explore mucociliary component. A multidisciplinary approach was developed to measure cilia beat patterns, viscoelasticity and movement of mucus. Methods: Human bronchial epithelial cells (HBEC) were obtained from endobronchial biopsies from patients with chronic respiratory diseases (asthma and COPD). HBEC were cultured in air/liquid interface for 28 days to obtain epithelium with a mucociliary phenotype. We measured epithelium thickness and cilia beat frequency (CBF) using fast videomicroscopy. Implication of mucus on CBF was assessed by measuring CBF before and after apical washes. We estimated the fraction of beating cilia and characterized mucus transport. Results. Thickness, CBF, cilia proportion and mucus transport coordination were obtained in control (n=4), mild asthma (n=3), severe asthma (n=10) and COPD (n=2) epithelia and showed that each group presented the same median CBF (f = 19 Hz). We emphasized a significantly stronger dispersion in CBF distribution in severe asthma group (Δf = 4.5 Hz) than in control group (Δf = 2.9 Hz). Conclusion. We showed that, on average, a smaller fraction of beating cilia was found in reconstituted epithelia of the severe asthma group in comparison with the control group. Moreover, a large dispersion is observed in the severe asthma group, with zones with no beating cilia and zones with a large number of beating cilia in a same reconstituted epithelium. Mucus involvement should be better investigated. ER -