@article {Ruiz4625, author = {Juan Jose Ruiz and Elena Puente and Mar{\'\i}a {\'A}ngeles Hoz and Jos{\'e} Luis Arroyo and Ram{\'o}n Ag{\"u}ero and Fernando Ausin and Maria Carmen Fari{\~n}as and Javier Gonzalo Ocejo-Vinyals}, title = {Relationship between a functional IL-17 single nucleotide polymorphism and susceptibility to pulmonary tuberculosis}, volume = {42}, number = {Suppl 57}, elocation-id = {4625}, year = {2013}, publisher = {European Respiratory Society}, abstract = {Background: Interleukin 17 (IL-17) plays an important role in immune response. Although low IL-17 levels have been associated with susceptibility to pulmonary tuberculosis (PTB), no study has evaluated if functional single nucleotide polymorphisms (SNPs) in IL-17A gene could influence risk of pulmonary tuberculosis.Objectives: To assess if rs2275913 (G-152A) SNP, a functional SNP located in the promoter region of the IL-17 gene, could be associated with susceptibility or resistance to pulmonary tuberculosis (PTB) in a genetically homogeneous population (Cantabria, Northern Spain).Methods: Through a case-control study, we studied the frequency of the different alleles and genotypes of the rs2275913 (G-152A) SNP. DNA obtained from one hundred and ninety-two patients with active PTB and 266 blood healthy donors was analysed by polimerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP).Results: The frequency of the GG versus GA + AA genotype was significantly more frequent in patients with PTB than in control subjects assuming a dominant model (50.52\% vs.39.10\% respectively, OR = 1.59, 95\% CI = 1.09-2.31, p = 0.015). Despite patients with PTB had higher serum IL-17 levels, we did not find an association of this SNP with IL-17 ex vivo production after stimulation with Mycobacterium tuberculosis (Mtb) antigens or phytohemagglutinin (PHA).Conclusions: Our results would suggest an association between IL-17A rs2275913 G allele and GG genotype with susceptibility to PTB for the first time.}, issn = {0903-1936}, URL = {https://erj.ersjournals.com/content/42/Suppl_57/4625}, eprint = {https://erj.ersjournals.com/content/42/Suppl_57/4625.full.pdf}, journal = {European Respiratory Journal} }