RT Journal Article
SR Electronic
T1 Multigene mutation analysis of mediastinal and hilar lymph nodes sampled by endobronchial unltrasound-guided needle aspiration
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 4638
VO 42
IS Suppl 57
A1 Thomas Vandemoortele
A1 Isabelle Nanni-Metellus
A1 Elise Kaspi
A1 Véronique Secq
A1 Sophie Laroumagne
A1 Patrice Roll
A1 Elisa Roca
A1 Hervé Dutau
A1 Philippe Astoul
A1 Andrée Robaglia
YR 2013
UL http://erj.ersjournals.com/content/42/Suppl_57/4638.abstract
AB Background: The presence of an Epidermal Growth Factor Receptor (EGFR) activating mutation guide treatment selection in advanced Non-Small Cell Lung Cancer (NSCLC), whereas (BRAF) mutations play a role in metastatic melanoma. We aimed to determine the feasibility of routine multigene mutation analysis for EGFR, KRAS, BRAF and PI3KCA in EndoBronchial UltraSound-guided (EBUS) TransBronchial Needle Aspiration (TBNA) in patients with NSCLC and melanoma.Methods: Data was prospectively collected from patients undergoing EBUS-TBNA from March 2011 until September 2012. Smears were studied after using Papanicolaou and May-Grünwald Giemsa stains. Genetic mutations were analyzed in metastatic lymph nodes diagnosed by EBUS TBNA in 40 patients with NSCLC and 1 melanoma patient. PCR and direct sequencing of EGFR exons 18 to 21, KRAS exon 2, BRAF exon 15 and PI3KCA exons 9 and 20 were performed.Results : Multigene mutation analysis was completed for samples from 40 patients (97,6%). Mutations were detected in 11 patients (26,8%) : 3 (7,3%) EGFR exon 19 deletion, 6 (14,6%) KRAS mutation, 1 (2,4%) BRAF, and 1 (2,4%) PI3KCA mutation. Only one sample could not be processed for the detection of KRAS mutation. All the samples were adequate for the other mutations detection. Molecular biology analyses were performed both on cytological samples and biopsy samples in 7 patients (17,1%), with a perfect correlation between these.Conclusions: Mutations of the EGFR, KRAS, BRAF and PI3KCA genes can be detected in cytological samples routinely obtained with EBUS TBNA.